Figure 3From: Pooled PCR testing strategy and prevalence estimation of submicroscopic infections using Bayesian latent class models in pregnant women receiving intermittent preventive treatment at Machinga District Hospital, Malawi, 2010Predictive relationship between pool size, number of tests and confidence intervals. Panel A: Expected point estimate and confidence interval for pools of sizes 2 through 30. Blue line and band represent point estimate and confidence interval for an imperfect test based on the values calculated in the LCM (True Prevalence: 8.0%, Sensitivity: 96.0%, Specificity: 99.1%). Confidence interval is not shown for pool sizes where confidence interval includes 100% prevalence (pool sizes >25). Green line and shaded area represent point estimate and confidence interval for a perfect test as would be assumed setting PCR as a gold standard (true prevalence 8.4%, sensitivity: 100%, specificity: 100%). Point estimate and confidence intervals for samples processed individually using imperfect test characteristics represented in black. Panel B: Expected number of tests required for one-step and two-step pooling strategies by pool size. Closed blue circle represents individual testing. Open blue circles represent expected number of tests from randomly-mixed one-step pools with an imperfect test. Closed green circles represent expected number of tests from two-step pools with an imperfect test. Reduction in number of tests relative to individual testing (individual tests/pooled tests) is represented for two-step pooling (green solid line) and one-step pooling (blue solid line).Back to article page