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Figure 5 | Malaria Journal

Figure 5

From: Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains

Figure 5

D and C affinity purified antibodies recognized polypeptides from the two allelic family of MSP2. Western Blot (WB) was performed using 3D7 and FC27 merozoites. A and B show respectively, specific 3D7- and FC27 D-individual adult pAbs (Burkina Faso, BF) against MSP2 allelic-derived polypeptides in WB, used at dilution of 1/100. Naive human sera (NHS) and C+ (pAb from 3D7-D + 8aa C) from Nigerian adult pool plasma were used as negative and positive controls, respectively. C) The C-terminal reactive-pAb from individual BF donor (that gave highest Ab titre in ELISA) was obtained to perform WB on both MSP2 allelic family parasites at a dilution of 1/100.

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Malaria Journal

ISSN: 1475-2875

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