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Table 2 MSP2 D and C domains epitope mapping in different age groups

From: Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains

 

Peptides

Children (1) (≤5 years) N = 38

Adolescents (2) (6–14 years) N = 43

Adults (3) (≥15 years) N = 26

p value

Positive: n (%)

Mean OD

SD

Positive: n (%)

Mean OD

SD

Positive: n (%)

Mean OD

SD

   
           

1 v 2

1 v 3

2 v 3

3D7 family

D

32 (84)

1.26

0.97

43 (100)

1.50

1.35

26 (100)

2.05

0.94

0.008

0.008

1

P11

0 (0)

0.11

0.01

2 (5)

0.12

0.02

8 (31)

0.17

0.11

0.148

0.001

0.007

P12

8 (21)

0.20

0.09

18 (42)

0.66

0.94

8 (31)

0.41

0.37

0.038

0.386

0.346

P13

16 (42)

0.64

0.95

20 (47)

0.44

0.73

11 (42)

0.40

0.37

0.690

0.987

0.733

P14

11 (29)

0.16

0.07

12 (28)

0.18

0.13

13 (50)

0.51

0.66

0.918

0.086

0.065

P15

13 (34)

0.18

0.13

24 (56)

0.39

0.53

9 (35)

0.59

0.83

0.045

0.973

0.078

P16

13 (34)

0.43

0.54

19 (44)

0.67

0.93

12 (46)

0.53

0.70

0.356

0.337

0.874

P17

5 (13)

0.20

0.16

22 (51)

0.21

0.16

7 (27)

0.29

0.35

<0.0001

0.181

0.036

P18

2 (5)

0.14

0.08

2 (5)

0.13

0.02

6 (23)

0.33

0.56

0.899

0.048

0.038

P19

14 (37)

0.22

0.31

4 (9)

0.11

0.01

2 (8)

0.20

0.27

0.002

0.002

0.814

FC27 family

D

23 (61)

0.21

0.11

37 (86)

0.85

1.15

23 (88)

0.76

0.50

0.007

0.006

0.768

P23

2 (5)

0.11

0.02

12 (28)

0.50

0.94

9 (35)

0.15

0.06

0.003

0.003

0.562

P24

0 (0)

0.14

0.04

4 (9)

0.45

0.83

7 (27)

0.21

0.13

0.036

0.002

0.071

P25

4 (11)

0.13

0.05

7 (16)

0.20

0.17

10 (38)

0.19

0.12

0.444

0.009

0.045

P26

4 (11)

0.11

0.02

10 (23)

0.18

0.17

8 (31)

0.17

0.08

0.118

0.050

0.499

P27

6 (16)

0.21

0.36

6 (14)

0.11

0.02

2 (8)

0.20

0.29

0.817

0.305

0.400

C region

C

14 (37)

0.27

0.28

19 (44)

0.25

0.12

14 (54)

0.50

0.40

0.500

0.175

0.435

P28

6 (16)

0.17

0.21

2 (5)

0.13

0.02

3 (23)

0.19

0.22

0.098

0.473

0.038

P29

5 (13)

0.12

0.04

11 (26)

0.13

0.04

8 (31)

0.18

0.11

0.150

0.096

0.644

P30

2 (5)

0.13

0.05

5 (12)

0.13

0.03

7 (27)

0.28

0.26

0.296

0.022

0.125

  1. Table 2 showed ELISA performed on LSPs and 20 mers covering the D and C fragments of the two allelic families of MSP2 using sera from age groups living in Mali. Plasma was used at dilution 1/200. The ELISA was considered positive if the ratio of mean Ab OD of test sample/mean OD of negative control was more than or equal to 2. Ab OD, SD and p-values from Fisher’s exact test (comparison of the proportions positive between two age groups) were calculated for each peptide. Numbers (1, 2, 3) represent respectively children, adolescents and adults code. v: versus. P values ≤ 0.05 are in bold and considered statically significant.