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Table 1 The putative merozoite cell surface and secreted proteins that were chosen for recombinant expression

From: Towards a comprehensive Plasmodium falciparum merozoite cell surface and secreted recombinant protein library

No

Name

Accession number

Type

Region targeted for recombinant expression

Exp. levels

1Â¥

rhoptry-associated membrane antigen (RAMA)

MAL7P1.208

GPI

Y17–S840

Medium

2

Prohibitin, putative

PF08_0006

Secreted

L20–F272

Low

3#

Conserved Plasmodium protein, unknown function

PF10_0166

Secreted

Y25–E310

High

4

GLURP

PF10_0344

Secreted

K24–I1233

Low

5

MSP3.5

PF10_0350

Secreted

A20–F710

High

6*

MSP3.6

PF10_0351

Secreted

N22–P566

Medium

7*

MSRP5

PF13_0191

Secreted

N22–I459

Medium

8*

MSRP4

MAL13P1.173

Secreted

D22–Q309

High

9Â¥

MSP8

PFE0120c

GPI

E26–S576

Low

10#

Conserved Plasmodium protein, unknown function

PF13_0125

Secreted

N20–S292

High

11

Conserved Plasmodium protein, unknown function

PF14_0044

Secreted

Q21–K290

N/D

12

Merozoite-associated tryptophan-rich antigen, putative

PFA0135w

Secreted

I25–K276

High

13

LCCL domain-containing protein

PFA0445w

Secreted

K22–I1029

N/D

14

SERA1

PFB0360c

Secreted

M1–V997

N/D

15Â¥

SERA2

PFB0355c

Secreted

E23–V1105

Medium

16

SERA3

PFB0350c

Secreted

T23–I930

Medium

17

SERA4

PFB0345c

Secreted

S26–V962

High

18

SERA5

PFB0340c

Secreted

T23–V997

High

19

SERA6

PFB0335c

Secreted

N25–V1031

Low

20Â¥

SERA7

PFB0330c

Secreted

Q23–V946

Low

21*

SERA9

PFI0135c

Secreted

E23–V932

Medium

22Â¥

Conserved Plasmodium protein, unknown function

PFA0210c

Secreted

Y24–D466

Low

23

Conserved Plasmodium protein, unknown function

PFB0475c

Secreted

L23–D446

Low

24

Cysteine-rich protective antigen (CyRPA)

PFD1130w

Secreted

D29–E362

High

25

RIPR

PFC1045c

Secreted

I20-N1086

Low

  1. Protein sequences from 3D7 reference P. falciparum strain were truncated to remove endogenous signal peptides and sequences corresponding to GPI anchors. The region of each protein that was targeted for recombinant expression is shown. Expression levels are given as a guide only because of the significant batch-to-batch variability of transient transfection, and grouped into "high" (between 30 μM and 100 μM after purification), "medium" (1 μM and 30 μM) and "low" (0.01 μM and 1 μM). No detectable expression (N/D) was obtained for PFA0445w, PF14_0044 and SERA1.
  2. ¥ Proteins selected from[51].
  3. * Proteins included to complete the set of proteins belonging to the MSP3, MSP7-like and SERA families.
  4. # Proteins chosen from[50]. All other proteins were short-listed from bioinformatic analysis of transcription microarray data[38, 39]. GPI, GPI-anchored.