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Table 3 Representative LDR-FM results for ten clinical samples

From: Validation of the ligase detection reaction fluorescent microsphere assay for the detection of Plasmodium falciparum resistance mediating polymorphisms in Uganda

Sample

Parasite density (per μl)

pfmdr1

pfcrt

  

86 N

86Y

184Y

184 F

1246D

1246Y

CVMNK

CVIET

SVMNT

1

12,560

3,386

106

3,600

209

2,018

101

1,235

231

174

2

74,080

1,849

1,885

2,083

2,284

3,286

309

811

1,678

195

3

35,120

812

2,937

177

4,031

4,140

365

232

2,115

195

4

8,960

3,761

188

2,879

3,361

4,536

164

207

2,311

214

5

24,560

4,081

125

4,068

322

3,386

93

162

2,438

163

6

1,440

143

3,777

4,024

292

115

2,065

222

2,430

164

7

25,520

1,502

3,754

3,928

1,788

2,496

1,881

304

2,440

163

8

36,064

2,829

2,846

311

5,227

3,148

937

1,119

1,610

211

9

18,320

2,677

132

3,154

555

4,254

143

1,097

1,262

243

10

14,840

4,121

100

3,686

267

196

1,715

1,385

317

231

  1. Values shown are uncorrected mean fluorescence readings. Readings representing the sequence call at each allele after correction by subtraction of background are in bold type.