Table 1 Primers and probes used in real-time PCR assay
From: A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR
Pfmdr1 | Â | Oligonucleotide (5'--> 3') | Tm** |
|---|---|---|---|
86 | F | TGTATGTGCTGTATTATCAGGAGGAAC | 63.1 |
| Â | R | AATTGTACTAAACCTATAGATACTAATGATAATATTATAGG | 61.7 |
| Â | Asn (wt*) probe | 6FAM-ACCTAAATT CATGTTCTTT-MGB-NFQ | 66.5 |
| Â | Tyr (mut**) probe | VIC-ACCTAAATA CATGTTCTTT-MGB-NFQ | 65.8 |
184 | F | AAGATGGACAATTTCATGATAATAATCCT | 59 |
| Â | R | AATACATAAAGTCAAACGTGCATTTTTTA | 57.6 |
| Â | Tyr (wt) probe | 6FAM-CTTTTTAGGTTTATA TATTTGGT-MGB-NFQ | 65.2 |
| Â | Phe (mut) probe | VIC-CTTTTTAGGTTTATT TATTTGGT-MGB-NFQ | 65.9 |
1034 | F | AAAAAGAAGAATTATTGTAAATGCAGCTT | 57.6 |
| Â | R | GGATCCAAACCAATAGGCAAAA | 58.9 |
| Â | Ser (wt) probe | 6FAM-ATTCA GTCAAAGCGCT-MGB-NFQ | 65.8 |
| Â | Cys (mut) probe | VIC-ATTCT GTCAAAGCGCT-MGB-NFQ | 66.7 |
1042 | F | AAAAAGAAGAATTATTGTAAATGCAGCTT | 57.6 |
| Â | R | TTTCCAGCATAACTACCAGTAAATATAAAAG | 60.7 |
| Â | Asn (wt) probe | 6FAM-CAATTATTTATTA ATAGTTTTGC-MGB-NFQ | 65.3 |
| Â | Asp (mut) probe | VIC-AATTATTTATTG ATAGTTTTGC-MGB-NFQ | 64.8 |