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Table 1 Oligonucleotide sequences used in the Hot Ligation

From: A simplified high-throughput method for pyrethroid knock-down resistance (kdr) detection in Anopheles gambiae

Description

Oligo Name

bp Positiona

Oligo sequence 5' – 3'

Modifications

Suspt. East kdr detector

Kdr104L-DTe

311-15i

ATTTGCATTACTTACGACTA

5' Biotin

Resist. East kdr detector

Kdr104S-DTe

311-15i

ATTTGCATTACTTACGACTG

5' Biotin

East kdr reporter

Kdr104-RTe

291–310

AATTTCCTATCACTACAGTG

5' Phosphorylation

3' Fluorescein

Suspt. West kdr detector

Kdr104L-DTw

312-16i

AATTTGCATTACTTACGACT

5' Biotin

Resist. West kdr detector

Kdr104F-DTw

312-16i

AATTTGCATTACTTACGACA

5' Biotin

West kdr reporter

Kdr104-RTw

292–311

AAATTTCCTATCACTACAGT

5' Phosphorylation

3' Fluorescein

  1. aUsing sequence from Martinez-Torres et al., as reference; i intron 2 position.