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Table 3 Comparison of results obtained with the SSOP-ELISA (horizontal) and RFLP (vertical). The single nucleotide polymorphisms (SNPs) in the HbB gene (top) and G6PD gene (bottom) were targeted using DNA extracted from control samples and 90 individuals from the field.

From: Rapid screening for glucose-6-phosphate dehydrogenase deficiency and haemoglobin polymorphisms in Africa by a simple high-throughput SSOP-ELISA method

HbB

SSOP-ELISA

 

RFLP

HbAA

HbAS

HbSS

HbAC

HbCC

No PCR prod.

 

HbAA

74

1

 

1

   

HbAS

 

11

     

HbSS

  

1

    

HbAC

   

1

   

HbCC

    

1

  

No PCR-prod.

     

3

 

G6PD

SSOP-ELISA

RFLP

68V

68M

68MV

126N

126D

126DN

No PCR prod.

68V

64

 

2

    

68M

 

5

     

68MV

 

2

8

    

126N

   

50

 

1

 

126D

    

14

  

126ND

    

2

16

 

No PCR-prod.

      

6

Inconclusive

4

 

2

2

 

2

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