Table 1 Primer sequence with corresponding annealing temperatures
Mouse Gene | GenBank Accession Number | Sequence (Forward) | Sequence (Reverse) | Annealing Temp (°C) |
|---|---|---|---|---|
RANTES | AF252285 | GGAAATCTTCGCACCTCAAG | GAGCGTGCGAACTTCTTGTT | 55 |
CCR1 | NM009912 | CCACTCCATGCCAAAAGACT | ATTAGGACATTGCCCACCAC | 50 |
CCR3 | NM009914 | TATCATTACCTGGGGCCTTG | CGAGGACTGCAGGAAAACTC | 53 |
CCR5 | D83648 | CGAAAACACATGGTCAAACG | GTTCTCCTGTGGATCGGGTA | 52 |
GAPDH | NM_008084 | CACAATTTCATCCCAGACC | GTGGGTGCAGCGAACTTTAT | 55 |