Table 1 PCR-RFLP analysis of msp-3α gene in 50 northern and 94 southern P. vivax clinical isolates from Iran. B = Belem Type 1; S =Salvador I Type 2; R = Recombinant Type 3 [20]. N = northern sample, S = southern sample. Different allelic variants obtained fromsequencing analysis of Iranian Pvmsp-1 gene grouped to Type 1 (B1-B9), Type 2 (S1-S16) and Type 3 (R1-R5). Within the same Typeall samples showed 100% similarity at both nucleotides and protein levels [20]. However, applying PCR-RFLP for genetic analysis ofidentical P. vivax samples by using Pvmsp-3α marker showed allelic variants within each Type.
Isolate Group | Amplified fragment size | Number of the allelic variant based on RFLP method | |||
|---|---|---|---|---|---|
Type A(1800 bp) | Type B(1500 bp) | Type C(1200 bp) | Hha I | Alu I | |
B1N | 3/37 (8.1%) | 2/37(5.4%) | 31/37(83.8%) | 4 | 4 |
B1S | - | - | 1/37 (2.7%) | 1 | 1 |
B2N | - | - | 2/2 (100) | 1 | 1 |
B2S | - | - | - | - | - |
B3N | - | - | - | - | - |
B3S | 3/3 (100%) | - | - | 2 | 2 |
B4N | - | - | - | - | - |
B4S | 1/1 (100%) | - | - | 1 | 1 |
B5N | - | - | - | - | - |
B5S | - | 1/1 (100%) | - | 1 | 1 |
B6N | - | - | - | - | - |
B6S | 2/2 (100%) | - | - | 1 | 1 |
B7N | - | - | - | - | - |
B7S | - | - | 2/2 (100%) | 2 | 2 |
B8N | - | - | - | - | - |
B8S | 3/3 (100%) | - | - | 1 | 1 |
B9N | - | - | - | - | - |
B9S | 3/3 (100%) | - | - | 2 | 2 |
S1N | 5/23 (21.7%) | 1/23 (4.3%) | - | 3 | 3 |
S1S | 13/23 (56.5%) | 2/23 (8.7%) | 2/23 (8.7%) | 7 | 7 |
S2N | 3/9 (34%) | - | 1/9 (11%) | 2 | 2 |
S2S | 1/9 (11%) | 2/9 (22%) | 2/9 (22%) | 3 | 3 |
S3N | - | - | 1/1 (100%) | 1 | 1 |
S3S | - | - | - | - | - |
S4N | - | - | - | - | - |
S4S | 1/1 (100%) | - | - | 1 | 1 |
S5N | - | - | - | - | - |
S5S | 2/2 (100%) | - | - | 1 | 1 |
S6N | - | - | - | - | - |
S6S | 1/1 (100%) | - | - | 1 | 1 |
S7N | - | - | - | - | - |
S7S | 1/1 (100%) | - | - | 1 | 1 |
S8N | - | - | - | - | - |
S8S | 2/2 (100%) | - | - | 1 | 1 |
S9N | - | - | - | - | - |
S9S | 5/5 (100%) | - | - | 1 | 1 |
S10N | - | - | - | - | - |
S10S | 3/3 (100%) | - | - | 2 | 2 |
S11N | - | - | - | - | - |
S11S | 1/1 (100%) | - | - | 1 | 1 |
S12N | - | - | - | - | - |
S12S | 2/9 (22.2%) | 1/9 (11.1%) | 6/9 (66.7%) | 2 | 2 |
S13N | - | - | - | - | - |
S13S | 1/2 (50%) | - | 1/2 (50%) | 2 | 2 |
S14N | - | - | - | - | - |
S14S | 1/1 (100%) | - | - | 1 | 1 |
S15N | - | - | - | - | - |
S15S | 2/2 (100%) | - | - | 2 | 2 |
S16N | - | - | - | - | - |
S16S | 1/1 (100%) | - | - | 1 | 1 |
R1N | 1/3 (33.4%) | - | - | 1 | 1 |
R1S | 2/3 (66.6%) | - | - | 2 | 2 |
R2N | - | - | - | - | - |
R2S | - | - | - | - | - |
R3N | - | - | - | - | - |
R3S | 2/4 (50%) | 1/4 (25%) | 1/4 (25%) | 3 | 3 |
R4N | - | - | - | - | - |
R4S | 2/2 (100%) | - | - | 2 | 2 |
R5N | - | - | - | - | - |
R5S | 13/17 (76.5%) | - | 4/17 (23.5%) | 4 | 4 |
Total (144) | 80 (55.5%) | 10 (7%) | 54 (37.5%) | 61 | 61 |