Figure 4

Fluorescence microscopy images illustrating RESA and propidium iodide staining of patient RBC. Thin blood smears were fixed with methanol and stained with either RESA Mab, propidium iodide or both. Images of the same field are shown in sets of three: a) green, b) red, and c) red + green composite. The number of RBCs showing pitted parasites (RESA+, panel A) were more than those with patent parasitaemia (PI+, panel B) and panel C. RBCs with pitted parasites showed other morphological abnormalities, including spherocyte formation (D).