Table 1 Microscopy "long" course training agenda
From: Establishing a malaria diagnostics centre of excellence in Kisumu, Kenya
Day | Time | Topic |
|---|---|---|
1 | 9.00 – 9.30 | Opening of the session |
9.30 – 10.30 | Introduction/Motivation/Goals of the course/Trials and tribulations | |
10.45 – 11.45 | Clinical presentation of malaria | |
11.45 – 12.45 | Qualities of a good microscopist | |
1.45 – 2.45 | Microscope maintenance | |
2.45 – 3.45 | Standard operating procedure (SOP) development | |
4.00 – 5.00 | Multiple choice questions (MCQ) written pre-test | |
Homework | Compare individual SOPs to Walter Reed Project SOPs. Note differences. | |
2 | 8.00 – 9.00 | Good Clinical Laboratory Practice/Quality control |
9.00 – 10.00 | Photographic image pre-test | |
10.15 – 1.15 | Slide reading pre-test (30 slides: 5 minutes each, 1 minute for passing the slide) | |
2.00 – 4.00 | Counting pre-test (12 slides: 10 minutes each) | |
4.15 – 5.15 | Review written test | |
Homework | Review Bench Aids for the diagnosis of malaria infections | |
3 | 8.00 – 10.00 | Slide preparation, review form for scoring slide quality |
10.15 – 11.15 | Malaria quality assurance & control | |
2.00 – 5.00 | Laboratory – Slide preparation and staining | |
4 | 8.00 – 9.30 | Species identification & groups' discussion |
9.45 – 12.45 | Laboratory – Species identification | |
1.45 – 2.45 | Approach to mixed infections | |
2.45 – 3.45 | Significance of false positives & negatives and counting | |
4.00 – 5.00 | Review photographic image test with discussion | |
5 | 8.00 – 9.30 | Artifacts, pseudo-parasites and the reality of false positives |
9.45 – 12.00 | Laboratory – Artifacts | |
1.00 – 3.45 | Laboratory – Slide reading, address individual difficulties | |
4.00 – 5.00 | SOPs – Groups' presentations and discussions | |
6, 7 | Weekend | Open microscopy laboratory |
8 | 8.00 – 9.00 | Malaria life cycle |
9.00 – 10.00 | Counting basics and techniques | |
10.15 – 12.00 | Laboratory – Practice counting using Walter Reed Project SOP | |
1.00 – 4.30 | Laboratory – Compare individual technique results to SOP technique results | |
4.45 – 5.15 | Review day 1 to 5 microscopy | |
9 | 8.00 – 9.00 | Low density infections |
9.00 – 9.30 | Cross contamination experiment | |
9.45 – 5.30 | Laboratory – Individual laboratory in reference to the pretest scores | |
10 | 8.00 – 9.00 | Malaria rereading paradigm |
9.00 – 10.00 | Review of the lectures | |
10.15 – 12.30 | Questions and trouble points, Panel discussion | |
1.30 – 5.00 | Laboratory – Review and scoring quality of blood slides | |
11 | 8.00 – 10.30 | Slide reading post-test |
10.45 – 11.45 | Written post-test | |
11.00 – 12.15 | Photographic image post-test | |
1.00 – 3.00 | Counting post-test | |
3.00 – 5.00 | Laboratory – Open microscopy and individual help | |
12 | 8.00 – 2.00 | Go over tests and results, give out take-home training materials |