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Table 2 Comparison of fluorescent dyes for dual staining of pRBCs in combination with Giemsa staining.

From: The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria

Fluorescent dye

Detection filter

Giemsa and fluorescent dye (light/fluorescence)*

SYBR Green 1

fluorescein

+/+

Ethidium monoazide (EMA)

rhodamine

 

   non photoactivated

 

+/-

   photoactivated

 

+/-

Yoyo-1

fluorescein

+/+

Acridine Orange

rhodamine

+/-

Ethidium homodimer-2 [EthD-2])

"

+/+

SYTO 44

fluorescein

+/-

SYTO 45

"

+/-

SYTO 17

rhodamine

+/-

SYTO 59

"

+/-

SYTO 61

"

+/-

SYTO 64

"

+/-

GelRed

"

+/-

Hoescht 33258

DAPI

+/-

DAPI

"

+/-

  1. *Plus/minus detection of Giemsa or fluorescence