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Figure 1 | Malaria Journal

Figure 1

From: A method for visualizing surface-exposed and internal PfEMP1 adhesion antigens in Plasmodium falciparum infected erythrocytes

Figure 1

Specific fluorescent antibody labelling of surface antigens on live P. falciparum -infected erythrocytes selected for cytoadhesion. All plates in the odd numbered plates show the DIC shadow-cast image of with the fluorescence image superimposed. All even numbered plates show the fluorescence image alone. The a series shows antibody binding to unselected P. falciparum FCR3 infected erythrocytes. Plates in the b series show P. falciparum FCR3 infected erythrocytes which have been pre-selected for adhesion to BeWo cells. Before preparation for microscopy magnetically purified cells were seeded with control uninfected erythrocytes. The antibodies used in each row of images are indicated on the left. Scale bar 5 μM.

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