From: Heterologous expression of plasmodial proteins for structural studies and functional annotation
PlasmoDB annotation | Product | Drug-ability Index (max is 1) | MM (Da) | pI | Heterologous Expression (Ref) | Tag | Special conditions (e.g. codon adaptation, TM, trunctions) | Structure (PDB code) |
---|---|---|---|---|---|---|---|---|
PF14_0053 | Ribonucleotide reductase small subunit | 1 | 40600 | 5.2 | E. coli[212] | His and GST | - | - |
PFD0830w | Bifunctional dihydrofolate reductase-thymidylate synthase | 1 | 71738 | 7.2 | - E. coli (various) - S. cerevisiae (complemeted) (various) - Cell free systems [207] | - | - Purposely truncated versions or domains - Codon optimised - S. cerevisiae adapted | 1J3I 1J3J 1J3K |
PFE0520c | Topoisomerase I | 1 | 98110 | 9.8 | - | - | - | - |
PFF0160c | Dihydroorotate dehydrogenase, mitochondrial precursor | 1 | 65559 | 9.4 | E. coli[213] | His | N-terminally truncated pRIL tRNA additions | 1TV5 |
PFI1020c | Inosine-5'-monophosphate dehydrogenase | 1 | 56151 | 8.0 | - | - | - | - |
PF11_0377 | Casein kinase 1 | 0.9 | 37631 | 9.7 | E. coli[214] | His | Â | 1lhx |
PF14_0192 | Glutathione reductase | 0.9 | 56679 | 8.1 | E. coli complementation [215] | His | Gene complementation | 1ONF |
PFI1685w | cAMP-dependent protein kinase catalytic subunit | 0.9 | 40197 | 9.1 | - | - | - | - |
PFI1170c | Thioredoxin reductase | 0.9 | 59687 | 7.8 | E. coli[216] | His | - | - |
MAL13P1.279 | Protein kinase 5 | 0.9 | 32997 | 8.0 | E. coli[217] | His | pRIL tRNA addition | 1OB3 |
PFC0525c | Glycogen synthase kinase 3 | 0.9 | 51616 | 5.2 | E. coli[218] | V5/His-/thioredoxin | - | - |
PFL2250c | Rac-beta serine/threonine kinase | 0.9 | 88096 | 10 | E. coli[219] | His or GST | pRIL tRNA addition | - |
PF10_0121 | Hypoxanthine phosphoribosyltransferase | 0.8 | 70076 | 4.6 | E. coli[220] | - | - | 1CJB |
PF10_0165 | DNA polymerase delta catalytic subunit | 0.8 | 127072 | 8.7 | E. coli[221] | 3-galactosidase | - | - |
PF10_0322 | S-adenosylmethionine decarboxylase-ornithine decarboxylase | 0.8 | 168171 | 6.3 | E. coli[222] | STREP | - | - |
PF13_0141 | L-lactate dehydrogenase | 0.8 | 34108 | 7.6 | - | - 1 TM/1 signal peptide - 2xYT - reducing cell growth temperature to 15°C | 1CEQ | |
PF13_0287 | Adenylosuccinate synthetase | 0.8 | 50066 | 7.7 | E. coli[225] | - | Low-temperature inductions were carried out at 20°C for 12 h | 1P9B |
PFI1090w | S-adenosylmethionine synthetase | 0.8 | 44844 | 6.7 | E. coli[226] | His | Â | - |
PF14_0076 | Plasmepsin 1 precursor | 0.8 | 51461 | 7.2 | Â | Â | 1 TM | 1PFZ |
PF14_0077 | Plasmepsin 2 precursor | 0.8 | 51481 | 5.3 | E. coli[227] | Â | - 1 TM - Solubilised and refolded | 1SME |
PF14_0125 | Deoxyhypusine synthase | 0.8 | 57429 | 6 | P. vivax expressed | Â | 1 TM | - |
PF14_0127 | N-myristoyltransferase | 0.8 | 47971 | 8.3 | E. coli[228] | His | - | -. |
PF07_0029 | Heat shock protein 86 | 0.8 | 86167 | 4.7 | E. coli[229] | His | - | - |
PF14_0164 | NADP-specific glutamate dehydrogenase | 0.8 | 52547 | 7.3 | - | - | 2bma | |
PF14_0378 | Triose-phosphate isomerase | 0.8 | 27935 | 6.4 | E. coli[232] | - | - | 1lyx |
PFD0590c | DNA polymerase alpha | 0.8 | 225404 | 8.4 | - | - | - | - |
PFE1050w | Adenosylhomocysteinase(S-adenosyl-L-homocystein e hydrolase) | 0.8 | 52840 | 5.7 | - | - | 1v8b | |
PFF0730c | Enoyl-acyl carrier reductase | 0.8 | 49763 | 9.5 | E. coli[235] | MBP | pRIL tRNA additions | 1nhg |
PFI1260c | Histone deacetylase | 0.8 | 51376 | 6.3 | Cell free systems [236] | No | - | - |
PFI1105w | Phosphoglycerate kinase | 0.8 | 45427 | 8.1 | E. coli[237] | His | - | 1lgi |
PFD1050w | Alpha-tubulin II | 0.8 | 49878 | 4.6 | - | - | - | - |
PFD0420c | Flap exonuclease 1 | 0.8 | 76868 | 8.1 | - | - | - | - |