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Table 1 Heterologous expression of 32 potential antimalarial drug targets.

From: Heterologous expression of plasmodial proteins for structural studies and functional annotation

PlasmoDB annotation

Product

Drug-ability Index (max is 1)

MM (Da)

pI

Heterologous Expression (Ref)

Tag

Special conditions (e.g. codon adaptation, TM, trunctions)

Structure (PDB code)

PF14_0053

Ribonucleotide reductase small subunit

1

40600

5.2

E. coli[212]

His and GST

-

-

PFD0830w

Bifunctional dihydrofolate reductase-thymidylate synthase

1

71738

7.2

- E. coli (various)

- S. cerevisiae (complemeted) (various)

- Cell free systems [207]

-

- Purposely truncated versions or domains

- Codon optimised

- S. cerevisiae adapted

1J3I

1J3J

1J3K

PFE0520c

Topoisomerase I

1

98110

9.8

-

-

-

-

PFF0160c

Dihydroorotate dehydrogenase, mitochondrial precursor

1

65559

9.4

E. coli[213]

His

N-terminally truncated pRIL tRNA additions

1TV5

PFI1020c

Inosine-5'-monophosphate dehydrogenase

1

56151

8.0

-

-

-

-

PF11_0377

Casein kinase 1

0.9

37631

9.7

E. coli[214]

His

 

1lhx

PF14_0192

Glutathione reductase

0.9

56679

8.1

E. coli complementation [215]

His

Gene complementation

1ONF

PFI1685w

cAMP-dependent protein kinase catalytic subunit

0.9

40197

9.1

-

-

-

-

PFI1170c

Thioredoxin reductase

0.9

59687

7.8

E. coli[216]

His

-

-

MAL13P1.279

Protein kinase 5

0.9

32997

8.0

E. coli[217]

His

pRIL tRNA addition

1OB3

PFC0525c

Glycogen synthase kinase 3

0.9

51616

5.2

E. coli[218]

V5/His-/thioredoxin

-

-

PFL2250c

Rac-beta serine/threonine kinase

0.9

88096

10

E. coli[219]

His or GST

pRIL tRNA addition

-

PF10_0121

Hypoxanthine phosphoribosyltransferase

0.8

70076

4.6

E. coli[220]

-

-

1CJB

PF10_0165

DNA polymerase delta catalytic subunit

0.8

127072

8.7

E. coli[221]

3-galactosidase

-

-

PF10_0322

S-adenosylmethionine decarboxylase-ornithine decarboxylase

0.8

168171

6.3

E. coli[222]

STREP

-

-

PF13_0141

L-lactate dehydrogenase

0.8

34108

7.6

E. coli[223, 224]

-

- 1 TM/1 signal peptide

- 2xYT

- reducing cell growth temperature to 15°C

1CEQ

PF13_0287

Adenylosuccinate synthetase

0.8

50066

7.7

E. coli[225]

-

Low-temperature inductions were carried out at 20°C for 12 h

1P9B

PFI1090w

S-adenosylmethionine synthetase

0.8

44844

6.7

E. coli[226]

His

 

-

PF14_0076

Plasmepsin 1 precursor

0.8

51461

7.2

  

1 TM

1PFZ

PF14_0077

Plasmepsin 2 precursor

0.8

51481

5.3

E. coli[227]

 

- 1 TM

- Solubilised and refolded

1SME

PF14_0125

Deoxyhypusine synthase

0.8

57429

6

P. vivax expressed

 

1 TM

-

PF14_0127

N-myristoyltransferase

0.8

47971

8.3

E. coli[228]

His

-

-.

PF07_0029

Heat shock protein 86

0.8

86167

4.7

E. coli[229]

His

-

-

PF14_0164

NADP-specific glutamate dehydrogenase

0.8

52547

7.3

E. coli[230, 231]

-

-

2bma

PF14_0378

Triose-phosphate isomerase

0.8

27935

6.4

E. coli[232]

-

-

1lyx

PFD0590c

DNA polymerase alpha

0.8

225404

8.4

-

-

-

-

PFE1050w

Adenosylhomocysteinase(S-adenosyl-L-homocystein e hydrolase)

0.8

52840

5.7

E. coli[233, 234]

-

-

1v8b

PFF0730c

Enoyl-acyl carrier reductase

0.8

49763

9.5

E. coli[235]

MBP

pRIL tRNA additions

1nhg

PFI1260c

Histone deacetylase

0.8

51376

6.3

Cell free systems [236]

No

-

-

PFI1105w

Phosphoglycerate kinase

0.8

45427

8.1

E. coli[237]

His

-

1lgi

PFD1050w

Alpha-tubulin II

0.8

49878

4.6

-

-

-

-

PFD0420c

Flap exonuclease 1

0.8

76868

8.1

-

-

-

-

  1. The TDR Targets database (tdrtargets.org) were searched for P. falciparum genes with a druggability evidence index ≥0.7. Hypothetical or putative proteins were excluded.
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Malaria Journal

ISSN: 1475-2875

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