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Table 1 Variance components of sperm and wing length in A. gambiae and A. stephensi males.

From: Intra-specific variation of sperm length in the malaria vector Anopheles gambiae: males with shorter sperm have higher reproductive success

Experiment 1: A. gambiae sperm length (μm)

Comp

df

SS

MS

V (μm2)

V (%)

F

P

male

24

3636431

151518

1012

10.7

4.98

< 0.001

testis

25

760304

30412

365

3.8

2.97

< 0.001

field

229

2345723

10243

206

2.2

1.30

0.002

sperm

2666

21031527

7889

7906

83.3

  

total

2944

27773985

 

9489

100.0

  

Experiment 1: A. stephensi sperm length (μm)

Comp

df

SS

MS

V (μm2)

V (%)

F

P

male

16

1530523

95658

703

10.9

4.12

0.003

testis

17

394867

23227

275

4.3

3.05

< 0.001

field

178

1355025

7612

251

3.9

1.46

< 0.001

sperm

1854

9682493

5222

5221

80.9

  

total

2065

12962908

 

6451

100.0

  

Experiment 2: A. gambiae wing length (mm)

Comp

df

SS

MS

V (μm2)

V (%)

F

P

family

31

0.58963

0.01902

3160.6

32.7

3.97

< 0.001

plate

32

0.153325

0.004791

0.0

0.0

0.65

0.911

male

64

0.45960

0.00741

6508.5

67.3

  

total

127

1.202555

 

9669.1

100.0

  

Experiment 2: A. gambiae sperm length (μm)

Comp

df

SS

MS

V (μm2)

V (%)

F

p

family

31

1655126

53391

62

0.8

1.30

0.231

plate

32

1312648

41020

50

0.6

1.14

0.325

male

64

2308407

36069

577

7.4

4.99

< 0.001

field

512

3700502

7228

10

0.1

1.01

0.407

sperm

5760

41045231

7126

7126

91.1

  

total

6399

50021914

 

7824

100.0

  

Experiment 3: A. gambiae wing length (mm)

Comp

df

SS

MS

V (μm2)

V (%)

F

p

family

15

0.071471

0.004765

140.8

3.1

1.06

0.416

male

44

0.197288

0.004484

4420.9

96.9

  

total

59

0.268759

 

4561.8

100.0

  

Experiment 3: A. gambiae sperm length (μm)

Comp

df

SS

MS

V (μm2)

V (%)

F

p

family

15

338142

22543

0

0.0

0.84

0.634

male

45

1212059

26935

354

5.0

3.31

< 0.001

field

244

1985344

8137

150

2.1

1.23

0.012

sperm

2745

18216008

6636

6636

92.9

  

total

3049

21751553

 

7140

100.0

  
  1. Experiments 1, 2, and 3: the variance in sperm length (μm) and male wing length (mm) was partitioned into various components (family, tissue culture plate, male, testis, field of view and sperm). For each component the degrees of freedom (df), sum of squares (SS), mean square (MS), the variance (V) with units of μm2 or as a percent (%), the F-statistic (F), and the statistical significance (p) are shown.