Figure 2

P. yoelii in vitro inhibition of DC maturation is dose dependent. DCs were incubated alone (Control), in the presence of uninfected RBCs (RBCs) or P. yoelii-infected erythrocytes (iRBCs) at the indicated ratio to DCs for 24 h followed by an additional 24 h in the presence or absence of 100 ng/ml LPS. FACS plots show CD40 and CD86 surface expression on DCs from control cultures (gray filled histograms) or incubated with LPS (black thick line). Mean fluorescent intensity (MFI) values are indicated in each plot for control and LPS stimulated. Each FACS plot is representative of one of at least 2 independent experiments.