Figure 2

Detection of expression of miR-451 by Northern blot. (A) Analysis of miR-451 expression in human WBCs and P. falciprum -iRBCs. Total RNA (2 μg) from WBCs and P. falciprum-iRBCs was separated by denaturing PAGE and blotted to a nylon membrane. Biotin-labeled miR-451 was used as a hybridization probe. Lane 1, infected RBC; Lane 2, isolated WBCs. (B) Analysis of miR-451 expression in different erythrocytic-stage P. falciparum -iRBCs. Total RNA (2 μg) from known numbers of cells were prepared at 24 h intervals from a single tightly synchronized culture. Parasite stage was determined by thin blood smears. Lane 1, healthy human RBCs; lane 2, 0-h culture after tight synchronization (ring-stage iRBCs); lane 3, 24-h culture (late trophozoite-stage iRBCs); lane 4, 48-h culture (schizont-stage iRBCs); lane 5, 72-h culture (new reproductive cycle of P. falciparum); lane 6, purified P. falciparum; lane 7, purified P. falciparum treated with RNase H to remove residual RNA contamination; lane 8, 72-h culture of P. falciparum-iRBCs loaded with equivalent numbers of erythrocytes as lane 2.