Immunofluorescence assays (IFA) for the localization of enolase at different stages of Plasmodium life cycle. (A) Asexual stages, (B) Gametocytes, (C) Gamete (mosquito stage) and (D) Sporozoite (mosquito salivary gland stage). Sporozoite preparation was from P. yoelii, while all other stages shown are for P. falciparum. Ring stages (triangle), trophozoite (*), schizont (→) and stage II gametocyte (diamond). Different fluorescent probes used were, (i) DAPI as a nuclear marker, (ii) mouse anti-r-Pfen (green), (iii) rabbit anti-r-Pfen (red), (iv) mouse anti-Pfg-27 (green) as gametocyte marker, (v) rabbit anti-male gametocyte specific α-tubulin II antibody (red) and (vi) mouse anti-CSP antibody (green) as sporozoite surface marker. The scale bars shown corresponds to 5 μm.