(A) Comparison of the distribution of enolase, aldolase and actin in soluble and particulate fractions prepared from P. falciparum cells in sexual and asexual stages. Cells were treated with 0.5% Triton X-100 for 10 minutes at 4°C. Solubilized proteins were removed by centrifugation (10,000 g for 30 minutes). Soluble (S) and particulate (P) fractions were analyzed on 12% SDS-PAGE and the presence of enolase was detected by Western blotting. (B) IFA of P. falciparum gametocyte stages (a, b, c) and asexual schizont stage (d). Cells were fixed either after a treatment with 1% Triton X-100 for 10 minutes (for the removal of cytosolic and membrane proteins) or without Triton treatment. Fixed cells were stained with rabbit or mouse anti-r-Pfen antisera along with (a) rabbit anti-P. falciparum aldolase (red), (b) mouse anti Pfs48/45 (green), (c) rabbit anti-T. gondii actin antibody (red) and (d) schizont (asexual stage) stained with DAPI, anti-r-Pfen and anti-actin antibodies after detergent treatment.