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Table 3 Projected and observed restriction enzymes fragments

From: Redefining the expressed prototype SICAvar gene involved in Plasmodium knowlesi antigenic variation

Restriction Enzyme Fragment Sizes (kb)

Probe: PkH_051981

 

Projected

Observed

Difference

Spans Sequence

Gap(s)

Ban II

5.2

Yes

None

No

Eco RV

11.1

Yes

None

No

Hind III

9.8

23

13.2

1 and 2

Hpa I

8.7

18

9

1

Nci I

4.5

13

8.5

1

Probe: Exon 4

 

Projected

Observed

Difference

Spans Sequence

Gap(s)

Ban II

11.7

22

10

2

Eco RV*

>13.6

40+

?

1 and 2

Hind III

9.8

23

13.2

1 and 2

Hpa I

9.3

12

2.7

2

Nci I

4.5

13

8.5

1

  1. * The gDNA sequence downstream of the 205 SICAvar gene has not been resolved, thus the location of the next Eco RV site is unknown.