Figure 5

Detection of Plasmodium in field-caught mosquitoes. (A) Cytb-PCR showing a band at position 729 bp (lane 1), and PCR-RFLP showing two bands at positions 438 bp and 291 bp (lane 2) suggesting presence of P. falciparum in the sample. (B) Using nested PCR, the same sample after first PCR showing a non-specific band at position 1.2 (lane 3) and after second PCR showing the specific band at position 205 bp (lane 4), concordantly suggesting presence of P. falciparum in the sample. The products were run on 2% agarose gel with 100 bp ladder as molecular size marker (M).