Differential gene expression mediated by 15-hydroxyeicosatetraenoic acid in LPS-stimulated RAW 264.7 cells

Schrimpe, Alexandra C; Wright, David W

doi:10.1186/1475-2875-8-195

Malaria Journal

Table 2 Select Gene Expression Changes Mediated by 15-HETE^a

From: Differential gene expression mediated by 15-hydroxyeicosatetraenoic acid in LPS-stimulated RAW 264.7 cells

Gene Symbol	Fold Change	Probe ID	Description	Entrez ID	Ref
Electron Transport [21]
Cyp3a11	3.6	516253	Cytochrome P450, family 3, subfamily a, polypeptide 11	13112
Smox	5.1	560410	Spermine oxidase	228608
Ugdh	1.9	500013	UDP-glucose dehydrogenase	22235
Regulation of Transcription, DNA-Dependent [21]
Bclaf1	-1.9	549609	BCL2-associated transcription factor 1	72567
Cdk9	2.1	392872	Cyclin-dependent kinase 9 (CDC2-related kinase)	107951
Creg1	1.9	760346	Cellular repressor of E1A-stimulated genes 1	433375
Egr1	-4.7	524988	Early growth response 1	13653
Fbxl11	-2.9	464056	F-box and leucine-rich repeat protein 11	225876
Fli1	2.3	407869	Friend leukemia integration 1	14247
Fliih	4.6	706377	Flightless I homologue (Drosophila)	14248
Hlx1	2.1	915372	H2.0-like homeo box 1 (Drosophila)	15284
Mxd1	2.1	520449	MAX dimerization protein 1	17119
Myst2	5.4	494053	MYST histone acetyltransferase 2	217127
Pou2f2	-4.4	911620	POU domain, class 2, transcription factor 2	18987
Pparbp	1.9	553770	Peroxisome proliferator activated receptor binding protein	19014
Pspc1	2.0	474771	Paraspeckle protein 1	66645
Rab11a	2.0	359489	RAB11a, member RAS oncogene family	53869
Rnf4	2.5	567180	Ring finger protein 4	19822
Tsc22d3	2.0	700170	TSC22 domain family 3	14605
Zfp482	2.0	435236	Zinc finger protein 482	241322
Protein Biosynthesis [20, 21]
Eprs	4.9	455664	Glutamyl-prolyl-tRNA synthetase	107508
Protein Folding [21]
Clpx	2.4	733670	Caseinolytic peptidase X (E. coli)	270166
Hspa4	3.5	578003	Heat shock protein 4	15525
Ubiquitin Cycle [21]
Cul7	0.4	742757	Cullin 7	66515
Fbxo3	1.9	832607	F-box only protein 3	57443
Ube2l6	2.0	401185	Ubiquitin-conjugating enzyme E2L 6	56791	[16]
Intracellular Protein Transport [21]
Ap1s2	2.0	605927	Adaptor-related protein complex 1, sigma 2 subunit	108012
Arf3	4.4	652348	ADP-ribosylation factor 3	11842
Sort1	2.4	339169	Sortilin 1	20661
Response to Stress [21]
Mapk14	2.2	755610	Mitogen activated protein kinase 14	26416	[16]
Prdx1	2.1	530413	Peroxiredoxin 1	18477	[17]
Prdx6	-2.0	825043	Peroxiredoxin 6	11758
Defense Response [20, 21]
Bst1	2.1	837914	Bone marrow stromal cell antigen 1	12182
Klra18	2.5	806675	Killer cell lectin-like receptor, subfamily A, member 18	93970
Tapbp	2.0	928057	TAP binding protein	21356	[20]
Inflammatory Response [20, 21]
Abcb1a	2.6	677412	ATP binding cassette, sub-family B (MDR/TAP), member 1A	97570
Ca2	4.0	574832	Carbonic anhydrase 2	88269
Card12	2.2	336709	Caspase recruitment domain family, member 12	268973
Cdkn1b	3.7	516253	Cytochrome P450, family 3, subfamily a, polypeptide 11	104565
Clu	4.0	379462	Clusterin	88423
Cr1l	1.9	538208	Complement component (3b/4b) receptor 1-like	88513
Cyp3a11	3.7	516253	Cytochrome P450, family 3, subfamily a, polypeptide 11	88609
Fyn	2.50	766362	Fyn proto-oncogene	95602
H2-Q8	1.8	712519	Histocompatibility 2, Q region locus 8	95937
Hnrnpa3	4.8	903894	Heterogeneous nuclear ribonucleoprotein A3	1917171
Mrc1	2.9	331550	Mannose receptor, C type 1	97142
Pole4	2.3	508321	Polymerase (DNA-directed), ε 4 (p12 subunit)	1914229
Ppp3r1	2.7	716541	Protein phospatase 3, regulatory subunit B, α isoform (calcineurin B, type I)	107172
Procr	2.0	431405	Protein C receptor, endothelial	104596
Rrm1	5.6	865694	Ribonucleotide reductase M1	98180
Serpinb2	4.0	860577	Serine (or cysteine) proteinase inhibitor, clade B, member 2	97609
Leukocyte Extravasation and Signalling
Arhgap12	2.2	465731	ρ GTPase activating protein 12	1922665
Crkl	3.0	389169	V-crk sarcoma virus CT10 oncogene homologue (avian)-like	104686
Ptk2b	2.6	867483	PTK2 protein tyrosine kinase 2 β	104908
Immune Response [20, 21]
Cxcl11	5.1	921243	Chemokine (C-X-C motif) ligand 11	56066
Ddx58	7.1	438990	DEAD (Asp-Glu-Ala-Asp) box polypeptide 58	230073
Fcgrt	2.0	390657	Fc receptor, IgG, α chain transporter	14132	[16]
Gbp1	2.9	586296	Guanylate nucleotide binding protein 1	14468	[16, 18, 20]
Gbp3	3.0	405120	Guanylate nucleotide binding protein 3	55932	[17, 20]
Ifit3	3.2	888038	Interferon-induced protein with tetratricopeptide repeats 3	15959
Il1a	5.1	595893	Interleukin 1 α	96542
Il1b	2.4	734612	Interleukin 1 β	16176
Il15	1.9	876196	Interleukin 15	16168
Cell Cycle [20]
Ccnf	2.4	767163	Cyclin F	12449
Cdkn1b	3.7	704876	Cyclin-dependent kinase inhibitor 1B (P27)	12576
Pmp22	1.9	616997	Peripheral myelin protein	18858
Rhob	-2.2	925472	Ras homologue gene family, member B	11852
Cell Adhesion [21]
Cldn11	4.9	338333	Claudin 11	18417
Scarb2	2.5	561450	Scavenger receptor class B, member 2	12492
Tnfaip6	1.9	614886	Tumor necrosis factor alpha induced protein 6	21930	[16]
Signal Transduction [20]
Ccr4	2.2	618105	Chemokine (C-C motif) receptor 4	12773	[18]
Cd86	3.2	908805	Cd86 antigen	12524	[20, 22]
Gnas	1.8	646267	GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus	14683
Ms4a4c	2.8	791872 495283	Membrane-spanning 4-domains, subfamily A, member 4C	64380
Olfr472	-2.2	591718	Olfactory receptor 472	258770
Prkrir	3.4	561755	Protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor)	72981
Ptger2	3.0	912597	Prostaglandin E receptor 2 (subtype EP2)	19217
Rin1	-1.9	478326	Ras and Rab interactor 1	225870
Ywhag	4.1	606287	3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide	22628
G-Protein Coupled Receptor Protein Signaling Pathway [21]
Olfr1303	-2.0	366625	Olfactory receptor 1303	258397
Olfr316	-1.9	903210	Olfactory receptor 316	258064
Olfr435	-3.2	810459	Olfactory receptor 435	258647
Slc19a2	1.9	763767	Solute carrier family 19 (thiamine transporter), member 2	116914
Cell-Cell Signaling [21]
Wnt6	2.0	590115	Wingless-related MMTV integration site 6	22420
Development [21]
Egfl4	-2.2	914308	EGF-like-domain, multiple 4	269878
Lrp6	-2.3	691244	Low density lipoprotein receptor-related protein 6	16974
Pgf	2.9	932795	Placental growth factor	18654
Metabolism [20]
Atp1a2	2.1	684165	ATPase, Na+/K+ transporting, α 2 polypeptide	98660
Atp2c1	2.0	388850	ATPase, Ca²⁺ sequestering	235574
Echdc3	2.2	331450	Enoyl Coenzyme A hydratase domain containing 3	67856
Hsd17b4	2.2	303973	Hydroxysteroid (17-beta) dehydrogenase 4	15488
Mmp9	-12.3	710293	Matrix metalloproteinase 9	17395
Oas3	-3.0	487213	2'-5' oligoadenylate synthetase 3	246727	[16]
Carbohydrate Transport [21]
Slc35a4	2.5	318829	Solute carrier family 35, member A4	67843
Protein Transport [21]
Exoc2	3.0	498825	Exocyst complex component 2	66482
Nupl2	2.1	868036	Nucleoporin like 2	231042
Rab20	2.0	410549	RAB20, member RAS oncogene family	19332
Rap2b	2.2	471908	RAP2B, member of RAS oncogene family	74012
Rheb	1.9	653270	RAS-homologue enriched in brain	19744
Zfyve20	2.9	669220	Zinc finger, FYVE domain containing 20	78287
Protein Ubiquitination [21]
Trim12	2.7	454451	Tripartite motif protein 12	76681
Trim34	2.9	600486	Tripartite motif protein 34	94094
Differentiation [21]
Ndrg2	2.1	468211	N-myc downstream regulated gene 2	29811
Other
Pik3ap1	2.0	646764	Phosphoinositide-3-kinase adaptor protein 1	83490	[16]

^aTranscripts altered ≥ 1.8-fold (p ≤ 0.025) in 15(S)-HETE-treated RAW 264.7 cells that are associated with specific genes or gene products correlated to malaria (referenced in column 6) or genes that are classified with specific over-expressed GO biological processes in malaria models (referenced with biological process heading), are shown in the table. Fold changes (FC) represent the average of three independent biological experiments. Bold FC indicate that multiple probes gave analogous results (average FC is shown).

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ISSN: 1475-2875

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