Haemoglobin interference and increased sensitivity of fluorimetric assays for quantification of low-parasitaemia Plasmodium infected erythrocytes

Table 1 Fluorescence of PG and SGI - DNA adducts on Plasmodium infected erythrocytes at low parasitaemia after removal of haemoglobin and detergents

	Fluorescence units (AU)
	SYBRGreen I^®	PicoGreen^®
% parasitaemia	IRBC	IRBC	IRBC-WBC	IRBC-WBC/W
5	15 ± 3	229 ± 12	243 ± 4	279 ± 8
2.5	9 ± 2	127 ± 7	141 ± 8	161 ± 7
1.3	6 ± 2	52 ± 1	83 ± 9	80 ± 4
0.6	5 ± 4	36 ± 3	36 ± 11	45 ± 2
0.3	ND¹	20 ± 5	20 ± 8	31 ± 4
0.1	ND	11 ± 3	11 ± 3	14 ± 2
0.05	ND	5 ± 4	ND	7 ± 3
Limits of detection and quantification (parasitaemia %)
LOD	1.37	0.23	0.21	0.24
LOQ	4.14	0.71	0.74	0.64

IRBC = Infected red-blood cells; IRBC-WBC = Infected red-blood cells whithout white blood cells removal; IRBC-WBC/W = Infected red-blood cells after white blood cells removal; ND = not detected.

ISSN: 1475-2875