Figure 3

Scatterplots of a parasite culture directly after synchronization (panel A), the same parasite culture after 24 h of incubation without drug (panel B) and the same parasite culture after 24 h of incubation with 1600 nM CQ (panel C). In drug-treated cultures, parasites do not progress to nuclear division and remain in the lower fluorescence intensity gate. Panel A also depicts the gating strategy used to distinguish between mononuclear and multinuclear parasitized cells in the FCMA. The number beside a gate denotes the percentage of the total events recorded in that gate.