Figure 2

Increased total sulphated glycosaminoglycan (sGAG) content following chloroquine exposure. In the placental cells, the sGAG content increased significantly following chloroquine (50 nM × 24 hours) (Panel A; p = 0.02, unpaired t-test, two-tailed). Similarly, the sGAG content increased linearly in response to exposure to increasing concentrations of chloroquine (Panel B; p < 0.0001, 1-way ANOVA for linear trend; r² = 0.92 for C38 cells and r² = 0.96 for IB3-1 cells). For the CVC, the increase in sGAG was significant following chloroquine (50 nM × 24 hours) and ASB siRNA (× 24 hours) (Panel C; p < 0.001, 1-way ANOVA with Tukey-Kramer post-test). [con si = control siRNA; ASB si = ASB siRNA; CVC = cerebrovascular cells; PC = placental cells; ASB = arylsulphatase B].