Figure 3

Increased chondroitin-4-sulphate (C4S) content following chloroquine exposure. The concentration of C4S increased significantly following exposure to increasing concentrations of chloroquine in the placental cells for 24 hours (Panel A; p = 0.002, unpaired t-test, two-tailed) and in the C38 and IB3-1 cells for 24 hours (Panel B; p < 0.0001, 1-way ANOVA for linear trend; r² = 0.96 for C38 cells and r² = 0.96 for IB3-1 cells). For the CVC cells, the increase in C4S was significant following chloroquine (50 nM × 24 hours) and ASB siRNA (× 24 hours) (Panel C; p < 0.001, 1-way ANOVA with Tukey-Kramer post-test). The decline in ASB activity was inversely correlated with the increase in C4S content (Panel D; r = 0.94). [con si = control siRNA; ASB si = ASB siRNA; CVC = cerebrovascular cells; PC = placental cells].