Figure 4

Development of anti-peptide IgG responses in ELISA after immunization with IRIV-formulated FB-12 in rabbits. Groups of three animals were immunized with two different doses (10 μg [A, C], and 50 μg [B, D] peptide-PE conjugate) without (A, B) or with (C, D) pre-immunization with 10 μg inactivated influenza virus (A/Sing). Shown are serial dilutions (means +/-SD) of rabbit sera taken two weeks after first, second and third immunization. No peptide-specific IgG responses were found in pre-immune sera.