Figure 1From: A novel method for standardized application of fungal spore coatings for mosquito exposure bioassaysK bar coating of fungal spores. (A) The stainless steel K-bar with wired grooves (inset) that control film thickness. (B) Automated (left) and manual K-Coater (right). (C) Application of fungal spore suspension on the centre top part of the paper substrate with a pipette. (D) Coating a paper substrate with spore suspension using a swift top-to-bottom movement of the bar using light pressure. (E) The mosquito exposure set-up with a coated paper inside a PVC tube (right), sealed with plastic foil. Mosquitoes were transferred into the holding tube with an aspirator and (after exposure) to the holding buckets (left) via free flight.Back to article page