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Table 1 Comparison of the effect of minimum particle size on the calculated prevalence of uninfected guts

From: A semi-automated method for counting fluorescent malaria oocysts increases the throughput of transmission blocking studies

Minimum particle size (pixels2)

1

2

3

4

6

8

Prevalence of 100 uninfected guts

as reported by the macro (%)

100

100

9   

7   

2   

2   

  1. Altering the minimum particle size used to identify oocysts by the counting macro affected the calculated prevalence of infection. 100 images of uninfected guts were counted by the macro using different minimum particle sizes. Due to many false positives, a minimum particle size of 1 to 2 pixels2 gave 100% prevalence. This dropped to 9% for 3 pixels2 and 2% by 6 pixels2.