Figure 8

Positive control images using endogenously expressed DsRED-tagged ACP instead of anti-ACP antibodies. The use of only one primary antibody, anti-PfSHMTc, with expressed DsRED tagged Pf ACP, was aimed at eliminating any possibility of artifactual fluorescence arising from interactions between two primary antibodies used simultaneously. (A) Two parasites, upper parasite is undergoing its first division, lower parasite is a late trophozoite. (B) Mitotic schizont with elongating apicoplast. (C) Mitotic schizont with ramifying apicoplast. All parasites show co-localization of anti-PfSHMTc fluorescence with the apicoplast, closely following the shape of the organelle, identical results to those obtained using two primary antibodies (scale bars (A) and (C) 3 μm, (B) 2 μm).