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Figure 5 | Malaria Journal

Figure 5

From: Uptake of purines in Plasmodium falciparum-infected human erythrocytes is mostly mediated by the human Equilibrative Nucleoside Transporter and the human Facilitative Nucleobase Transporter

Figure 5

Hypoxanthine uptake in uninfected and P. falciparum -infected human erythrocytes. A, Uptake of 1 μM [3H]-hypoxanthine into P. falciparum-infected erythrocytes (closed symbols and dotted lines) and uninfected erythrocytes (open symbols, solid lines), expressed as pmol(107 cells)-1, was determined for up to 10 s: squares, no inhibitor; circles, 250 μM furosemide; triangles, 1 mM unlabelled hypoxanthine. Lines were calculated by linear regression and the results are the average of triplicate determinations. B, Bargraph of uptake of 0.5 μM [3H]-hypoxanthine in P. falciparum-infected human erythrocytes over a 2 s interval, expressed as pmol(107 cells)-1s-1. Bars are: Control, no inhibitor added; NPPB. 300 μM NPPB; Hypox, 1 mM hypoxanthine added; Both, 300 μM NPPB plus 1 mM hypoxanthine added; Closed bars and open bars represent identical experiments with two different batches of human erythrocytes, from different donors. Bars are mean and SEM of triplicate determinations at room temperature.

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