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Table 2 Comparison of three DNA extraction methods for optimal recovery of DNA from unstained and stained TBFs and thin blood films.

From: Giemsa-stained thick blood films as a source of DNA for Plasmodium species-specific real-time PCR

 

QIAGEN method

Water method

Chelex method

 

Ct Pf

Ct HBB

Ct Pf

Ct HBB

Ct Pf

Ct HBB

unstained TBF

38,21

33,92

0

44,69

0

0

stained TBF

38,92

32,25

0

0

0

0

unstained thin blood film

39,43

35,32

0

40,19

0

0

stained thin blood film

39,90

34,80

0

0

0

0

  1. Cycle threshold (Ct)-values were given for the detection of parasite DNA (Pf: Plasmodium falciparum) and human DNA by human beta globin (HBB) PCR.