| | FCA | FCA | OM | OM |
---|
Experiment number | M1 (%) | Female Gam (%): M2 | Male Gam + Sch (%): M3 | Gam (%) | Sch (%) |
---|
1
| 36.9 | 14.7 | 47.9 | 74 | 2.1 |
2
| 66.9 | 2.5 | 28.7 | 38 | 19 |
3
| 85.1 | 6.6 | 8.5 | 18.9 | 0.5 |
4
| 80.9 | 12.6 | 6.4 | 13.4 | 0 |
5
| 83.3 | 3.5 | 13.3 | 17 | 0 |
6
| 90.9 | 5.8 | 2.5 | 6.9 | 1 |
7
| 93.6 | 1.6 | 4.6 | 3 | 1 |
mean
| 76.8 | 6.8 | 16.0 | 24.4 | 3.3 |
- Parasitaemias of in vitro P. falciparum gametocyte cultures (W2 strain) were evaluated by flow cytometry and optical microscopy (FCA: flow cytometry assay; OM: optical microscopy). These values correspond to the percentages of every population after gametocyte (Gam) enrichment by MACS.
- M1: percentage corresponding to the first peak of the histogram in FCA, which corresponds to non-fluorescent events (uninfected erythrocytes).
- M2: parasitaemia corresponding to the second peak of the histogram in FCA (erythrocytes infected by gametocytes).
- M3: parasitaemia corresponding to the third peak of the histogram in FCA (erythrocytes infected by gametocytes and/or residual asexual stages (schizonts: Sch)).