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Table 1 Comparison between FCA (Flow cytometry analysis) an OM (Optical microscopy) for the determination of gametocytaemia.

From: Flow cytometry for the evaluation of anti-plasmodial activity of drugs on Plasmodium falciparum gametocytes

  

FCA

FCA

OM

OM

Experiment number

M1 (%)

Female Gam

(%): M2

Male Gam

+ Sch (%): M3

Gam (%)

Sch (%)

1

36.9

14.7

47.9

74

2.1

2

66.9

2.5

28.7

38

19

3

85.1

6.6

8.5

18.9

0.5

4

80.9

12.6

6.4

13.4

0

5

83.3

3.5

13.3

17

0

6

90.9

5.8

2.5

6.9

1

7

93.6

1.6

4.6

3

1

mean

76.8

6.8

16.0

24.4

3.3

  1. Parasitaemias of in vitro P. falciparum gametocyte cultures (W2 strain) were evaluated by flow cytometry and optical microscopy (FCA: flow cytometry assay; OM: optical microscopy). These values correspond to the percentages of every population after gametocyte (Gam) enrichment by MACS.
  2. M1: percentage corresponding to the first peak of the histogram in FCA, which corresponds to non-fluorescent events (uninfected erythrocytes).
  3. M2: parasitaemia corresponding to the second peak of the histogram in FCA (erythrocytes infected by gametocytes).
  4. M3: parasitaemia corresponding to the third peak of the histogram in FCA (erythrocytes infected by gametocytes and/or residual asexual stages (schizonts: Sch)).