Figure 2

Effects of P.berghei infection on CYP activities, NO production and liver weights in C57BL/6 mice. Time course of changes of liver CYP activities (CYP1a: EROD, 2b: BROD, 2a5: COH), NO serum levels, and liver and spleen weights in C57BL/6 mice infected with P.berghei (ANKA). Panel A shows NO serum levels (μM) and parasitaemia rates (%PE). Results in Panel B (enzyme activities) and C (liver and spleen weights) are shown as % of mean values for non-infected controls (day 0 = 100) and comparisons were made by ANOVA and Dunnett's post-hoc test. In panels B and C statistical evaluation was made with untransformed data. Differences (P < 0.05) from non-infected control group (day 0) are indicated by an asterisk (*) superscript. Panels B and C control values (100%) are as follows (mean ± S.E.M.): EROD = 103.1 ± 33.5 pmol resorufin/mg protein/min; BROD = 87.3 ± 12.3 pmol resorufin/mg protein/min; COH = 9.81 ± 2.5 pmol umbelliferone/mg protein/min; liver wt = 0.99 ± 0.05 g; spleen wt = 0.09 ± 0.01 g. Control NO = 37.95 ± 2.4 μM (Panel A). Numbers of mice used (N) are as follows: PID0 = 5; PID2 = 7; PID4 = 7; PID6 = 7; PID8 = 7; PID10 = 3. Numbers of infected mice which died (not included): PIDs8/9 = 5, PIDs9/10 = 13. From day 8 onwards, all infected mice that were killed or died presented neurological signs of CM.