Remarkable diversity of intron-1 of the para voltage-gated sodium channel gene in an Anopheles gambiae/Anopheles coluzzii hybrid zone

Table 1 Intron-1 of VGSC gene polymorphism and summary statistics in Anopheles coluzzii and A. gambiae samples

	African region	n	H	Hd	S	π (%)	θ (%)	*Tajima D*	Fu & Li’s D	Fu & Li’s F
*A. coluzzii*
	Far-West	50	8	0.73	8	0.49	0.34	1.217	−0.127	0.361
	West	92	7	0.68	11	0.21	0.41	−1.303	0.120	−0.450
	Central	38	4	0.15	3	0.03	0.14	−1.720	−2.848*	−2.922*
*A. gambiae*
	Far-West	82	14	0.70	9	0.47	0.34	0.926	−0.123	0.277
	West	78	3	0.12	2	0.02	0.08	−1.121	−1.011	−1.218
	Central	46	8	0.51	7	0.12	0.30	−1.657	−2.583*	−2.686*
	East	16	4	0.65	6	0.22	0.34	−1.230	−2.025	−2.076
Hybrids
	Far-West	24	8	0.77	7	0.52	0.36	1.472	0.629	1.016

Data include original sequences and those from Gentile et al. [23]. Far-West = Senegal, Gambia, Guinea Bissau, Guinea Conakry; West = Mali, Ivory Coast, Burkina Faso, Benin, Nigeria; Central = Cameroon and Angola; East = Rwanda. n = n° alleles; H = n° haplotypes; S = n° segregating sites; Hd = haplotype diversity; π = nucleotide diversity; θ = Watterson estimate of theta; for Tajima D, Fu & Li’s D and F values, p computed using confidence levels provided by the coalescent (DNAsp 5.0) * < 0.05.

ISSN: 1475-2875