Prevalence of pfhrp2 and pfhrp3 gene deletions in Puerto Lempira, Honduras

Malaria Journal

Table 2 Deletion pattern of Pfhrp3 and its flanking genes in two population clusters

MAL13P1.475	*Pfhrp3* exon 1–2	MAL13P1.485	All samples, N (%)	Cluster 1	Cluster 2
+	+	+	34 (50%)	19 (70.4%)	13 (35.1%)
+	+	-	0 (0%)	0 (0.0%)	0 (0.0%)
-	+	+	3 (4.4%)	1 (3.7%)	2 (5.4%)
+	-	-	2 (2.9%)	0 (0.0%)	2 (5.4%)
-	-	+	18 (26.5%)	6 (22.2%)	12 (32.4%)
-	-	-	10 (14.7%)	1 (3.7%)	8 (21.6%)
+	-	+	0 (0%)	0 (0.0%)	0 (0.0%)
-	+	-	1 (1.5%)	0 (0.0%)	1 (2.7%)
Total			68	27*	38*

The symbol ‘+’ indicates gene amplification while ‘-’ indicates no gene amplification after two attempts. N – number of samples. *A total of 65 samples were included in the population cluster analysis using Structure v2.3.3. Three samples were excluded because more than one allele was amplified in one or more microsatellite loci, indicating possible multiple strain infection. The columns labeled ‘Cluster 1’ and ‘Cluster 2’ show the absolute numbers (and percentage proportions) of samples with the indicated MAL13P1.475/ Pfhrp3/ MAL13P1.485 gene profiles that were assigned to each of the two parasite population clusters identified using Structure v2.3.3.

ISSN: 1475-2875