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Figure 3 | Malaria Journal

Figure 3

From: The Plasmodium vivax rhoptry neck protein 5 is expressed in the apical pole of Plasmodium vivax VCG-1 strain schizonts and binds to human reticulocytes

Figure 3

r Pv RON5 expression and antigenicity studies. (A) rPvRON5 expression and purification. Lane 1, rPvRON5 purified by affinity chromatography with Coomassie blue staining. A single ~33 kDa band was observed which coincided with the expected molecular weight. Lane 2, WB recognition of rPvRON5 using anti-polyhistidine monoclonal antibody. (B) P. vivax patients’ sera reactivity with rPvRON5. Top panel, recognition of recombinant protein by WB. H: reactivity by monoclonal anti-polyhistidine serum. Lanes 1 to 11, recognition of rPvRON5 by sera from P. vivax-infected patients. A single ~33 kDa band can be seen. Lanes 12 to 15, sera from healthy individuals. R: Polyclonal serum 2 reactivity with rPvRON5. Bottom panel, ELISA recognition of rPvRON5 by sera from P. vivax-infected patients (grey bars) and healthy individuals (black bars).

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