Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas

Kerkhof, Karen; Canier, Lydie; Kim, Saorin; Heng, Somony; Sochantha, Tho; Sovannaroth, Siv; Vigan-Womas, Inès; Coosemans, Marc; Sluydts, Vincent; Ménard, Didier; Durnez, Lies

doi:10.1186/s12936-015-0868-z

Table 1 Overview of the antigens (peptides and recombinant proteins) used in this study

From: Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas

Antigens	Sequence (N-terminal to C-terminal)	g/mol	Life-cycle stages	Plasmodium species	Peptide or recombinant protein	References
CSP	NANPNANPNANPNANPNVDPNVDPC	2557.67	Sporozoite	P. falciparum	Peptide	[5]
Pfl3	C-terminal His-tag produced in E. coli		Sporozoite	P. falciparum	Recombinant protein	[45, 46]
STARP-R	STDNNNTKTISTDNNNTKTIC	2299.42	Sporozoite and liver stage	P. falciparum	Peptide	[5, 47]
SALSA 1	SAEKKDEKEASEQGEESHKKENSQESAC	3123.24	Sporozoite and liver stage	P. falciparum	Peptide	[5, 47]
SALSA 2	NGKDDVKEEKKTNEKKDDGKTDKVQEKVLEKSPKC	4019.52	Sporozoite and liver stage	P. falciparum	Peptide	[5, 47
SR11.1	EEVVEELIEEVIPEELVLC	2213.5	Sporozoite and liver stage	P. falciparum	Peptide	[5, 47]
LSA1-41	LAKEKLQEQQSDLEQERLAKEKLQEQQSDLEQERLAKEKEKLQC	5297.97	Liver stage	P. falciparum	Peptide	[5, 6, 48]
LSA1-J	ERRAKEKLQEQQSDLEQRKADTKKC	3046.43	Liver stage	P. falciparum	Peptide	[5, 47, 48]
LSA3-NR2	VLEESQVNDDIFNSLVKSVQQEQQHNVC	3230.53	Liver stage	P.falciparum	Peptide	[5, 47]
LSA3-RE	VESVAPSVEESVAPSVEESVAENVEESVC	2991.2	Liver stage	P. falciparum	Peptide	[5, 47]
PfMSPl-19	Glutathione S-transferase (GST) fusion protein. C-terminal expressed in E. coli		Merozoite	P. falciparum	Recombinant protein	[4, 24]
GLURP	EDKNEKGQHEIVEVEEILC	2241.47	Trophozoite	P. falciparum	Peptide	[5, 47, 49]
GLURP-P3	EPLEPFPTQIHKDYKC	1945.23	Trophozoite	P. falciparum	Peptide	[5, 47, 50]
PfGLURP-R2	C-terminal produced in E. coli		Trophozoite	P. falciparum	Recombinant protein	[49]
Pvlike CSP	APGANQEGGAAAPGANQEGGAAAPGANQEGGAAC	2892.99	Sporozoite	P. vivax	Peptide	[47]
PvVK210 CSP	DGQPAGDRAAGQPAGDRADGQPAGDRADGQPAGC	3206.3	Sporozoite	P. vivax	Peptide	[47, 51, 52]
PvVK247 CSP	ANGAGNQPGANGAGNQPGANGAGNQPGANGAGNC	2905.95	Sporozoite	P. vivax	Peptide	[47, 51, 52]
PvCSP-chimera	Soluble His-tag protein expressed in wheat-germ cell free expression system		Sporozoite	P. vivax	Recombinant protein	[51, 52]
PvAMAl			Merozoite	P. vivax	Recombinant protein	[53, 54]
PvDBP			Merozoite	P. vivax	Recombinant protein	[55]
PvMSPl-19	C-terminal produced in the baculovirus expression system		Merozoite	P. vivax	Recombinant protein	[4, 16, 24]
PmCSP	GNAAGNAAGNDAGNAAGNAAGNAAGNAAGNAAC	2358.37	Sporozoite	P. malariae	Peptide	[47]
SALIV 1	EKVWVDRDNVYCGHLDCTRVATFC	2830.22	Salivary gland proteins	An. gambiae	Peptide	[5, 56]
SALIV 2	ATFKGERFCTLCDTRHFCECKETREPLC	3324.84	Salivary gland proteins	An. gambiae	Peptide	[5, 56]

Ags are organized according to the Plasmodium species and the life-cycle stages in the human host

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ISSN: 1475-2875

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