Table 1 Means, standard deviations, and seropositivity cutoff values for each antigen as determined by different immunoassays and statistical methods
Distribution, assay | Antigen | Malaria-naïve reference population | Finite mixture model, first component | ||||
|---|---|---|---|---|---|---|---|
Mean (SD) | Mean + 3SD | Mean + 5SD | Mean (SD) | Mean + 3SD | Mean + 5SD | ||
Normal distribution | |||||||
 ELISA (in OD) | MSP1-42(D) | 0.045 (0.027) | 0.126 | 0.180 | 0.125 (0.069) | 0.332 | 0.470 |
MSP1-42(F) | 0.030 (0.019) | 0.087 | 0.125 | 0.127 (0.068) | 0.331 | 0.467 | |
MSP1-19 | 0.052 (0.025) | 0.127 | 0.177 | 0.157 (0.080) | 0.397 | 0.557 | |
AMA-1 | 0.056 (0.038) | 0.170 | 0.246 | 0.14 (0.069) | 0.347 | 0.485 | |
 Multiplex (in MFI) | MSP1-42(D) | 10.7 (21.2) | 74.3 | 116.7 | 18.3 (16.7) | 68.4 | 101.8 |
MSP1-42(F) | 16.4 (29.1) | 103.7 | 161.9 | 21.7 (22.4) | 88.9 | 133.7 | |
MSP1-19 | 11.1 (10.7) | 43.2 | 64.6 | 11.4 (10.5) | 42.9 | 63.9 | |
AMA-1 | 15.3 (19.0) | 72.3 | 110.3 | 21.7 (23.5) | 92.2 | 139.2 | |
Lognormal distribution | |||||||
 ELISA (in OD) | MSP1-42(D) | 0.045 (0.027) | 0.126 | 0.180 | 0.085 (0.572) | 1.801 | 2.945 |
MSP1-42(F) | 0.030 (0.021) | 0.093 | 0.135 | −1.214 (1.242) | 2.512 | 4.996 | |
MSP1-19 | 0.052 (0.026) | 0.130 | 0.182 | 0.377 (0.385) | 1.532 | 2.302 | |
AMA-1 | 0.056 (0.037) | 0.167 | 0.241 | 0.292 (0.500) | 1.792 | 2.792 | |
 Multiplex (in MFI) | MSP1-42(D) | 10.1 (17.0) | 61.1 | 95.1 | 2.5 (0.96) | 5.4 | 7.3 |
MSP1-42(F) | 15.3 (21.6) | 80.1 | 123.3 | 2.5 (0.69) | 4.6 | 6.0 | |
MSP1-19 | 10.9 (9.8) | 40.3 | 59.9 | 2.0 (0.84) | 4.5 | 6.2 | |
AMA-1 | 15.0 (16.6) | 64.8 | 98.0 | 2.5 (0.91) | 5.2 | 7.1 | |