- Open Access
A WD40-repeat protein unique to malaria parasites associates with adhesion protein complexes and is crucial for blood stage progeny
Malaria Journal volume 14, Article number: 435 (2015)
During development in human erythrocytes, Plasmodium falciparum parasites display a remarkable number of adhesive proteins on their plasma membrane. In the invasive merozoites, these include members of the PfMSP1 and PfAMA1/RON complexes, which facilitate contact between merozoites and red blood cells. In gametocytes, sexual precursor cells mediating parasite transmission to the mosquito vector, plasma membrane-associated proteins primarily belong to the PfCCp and 6-cys families with roles in fertilization. This study describes a newly identified WD40-repeat protein unique to Plasmodium species that associates with adhesion protein complexes of both merozoites and gametocytes.
The WD40-repeat protein-like protein PfWLP1 was identified via co-immunoprecipitation assays followed by mass spectrometry and characterized using biochemical and immunohistochemistry methods. Reverse genetics were employed for functional analysis.
PfWLP1 is expressed both in schizonts and gametocytes. In mature schizonts, the protein localizes underneath the merozoite micronemes and interacts with PfAMA1, while in gametocytes PfWLP1 primarily accumulates underneath the plasma membrane and associates with PfCCp1 and Pfs230. Reverse genetics failed to disrupt the pfwlp1 gene, while haemagglutinin-tagging was feasible, suggesting a crucial function for PfWLP1 during blood stage replication.
This is the first report on a plasmodial WD40-repeat protein associating with cell adhesion proteins. Since WD40 domains are known to mediate protein–protein contact by serving as a rigid scaffold for protein interactions, the presented data suggest that PfWLP1 supports the stability of adhesion protein complexes of the plasmodial blood stages.
Protein complexes are formed by two or more non-covalently bound proteins mutually supportive in distinct cell functions. Protein complexes are crucial for most cell biological processes and among others function in establishing cell–cell contacts. Thus such complexes are of importance for interactions between pathogens and between pathogens and their host cells.
For intracellular pathogens, such as malaria parasites, recognition, adhesion and invasion of host cells are essential steps during infection and are often mediated by protein–protein interactions. A variety of protein complexes have previously been described for the human malaria parasite Plasmodium falciparum, like the actin-myosin motor complex enabling gliding motility of the invasive stages. Also, most of the adhesive proteins found on the surface of the invasive merozoites involved in binding to the red blood cell (RBC) prior to infection are known to assemble as complexes, e.g., the merozoite surface protein 1 (MSP1) complex or the apical membrane antigen 1 (AMA1)/Rhoptry neck (RON)-complex (reviewed in [1–3]).
The large MSP complex consists mainly of the GPI-anchored surface protein MSP1, the peripheral membrane proteins MSP6 and MSP7 and the recently found MSP-Duffy-binding like proteins MSPDBL1 and MSPDBL2 (, reviewed in [2, 3]). Although the exact mechanistic function of the MSP1 complex is not fully understood, it is suggested that the MSP complex is responsible for the initial interaction of the merozoite with the RBC. The AMA1/RON complex, on the other hand, is crucial for the formation of the tight junction during merozoite invasion. It was shown that AMA1, a transmembrane protein of the micronemal membrane, upon merozoite attachment to the RBC relocates to the plasma membrane and then interacts with RON proteins that have been secreted and inserted into the RBC membrane. Here, RON2 functions as an anchor on the RBC membrane, interacting with the parasite transmembrane protein AMA1 and thereby forming the tight junction ([5, 6], reviewed in ).
Protein complexes can also be found in the non-invasive, amotile gametocyte stages of P. falciparum. Gametocytes are dormant sexual precursor cells of the malaria pathogen, which differentiate in human RBCs and once taken up during the blood meal, become activated and transform into male and female gametes to initiate sexual reproduction (reviewed in ). A remarkable feature of gametocytes is the expression of numerous adhesive proteins, which are associated with the plasma membrane within the parasitophorous vacuole. These include the six-cysteine (6-cys) proteins Pfs230 and Pfs48/45 and the six Limulus coagulation factor C-like (LCCL)-domain containing PfCCp proteins (reviewed in ). Noteworthy, the PfCCp proteins assemble to protein complexes associated with the gametocyte plasma membrane via an interaction with Pfs230, which itself is bound to the GPI-anchored Pfs48/45 ([10–13], reviewed in ). Recent findings demonstrated a rearrangement of this protein complex during gametogenesis resulting into an increased interaction of the PfCCp proteins to Pfs230 as well as to the GPI-anchored protein Pfs25 of macrogametes .
In an attempt to identify further interaction partners of the PfCCp-based protein complex, in this study co-immunoprecipitation assays were employed and identified a WD40-repeat protein unique to the Plasmodium species that associates with selected adhesion protein complexes of merozoites and gametocytes. Since WD40-repeat domains are known to facilitate protein–protein contact, the data point to the WD40-repeat protein playing a role in promoting stability of the cell adhesion protein complexes in these stages.
The following gene identifiers are assigned to the proteins investigated in this study: PfAMA1 [PlasmoDB: PF3D7_1133400]; PfCCp1 [PlasmoDB: PF3D7_1475500]; PfCCp2 [PlasmoDB: PF3D7_1455800]; PfCCp4 [PlasmoDB: PF3D7_0903800]; Pfs48/45 [PlasmoDB: PF3D7_1346700]; Pfs230 [PlasmoDB: PF3D7_0209000]; PfGAP45 [PlasmoDB: PF3D7_1222700]; PfGAP50 [PlasmoDB: PF3D7_0918000]; Pfα-tubulin I [PlasmoDB: PF3D7_0903700]; PfEXP1 [PlasmoDB: PF3D7_1121600]; PfMSP1 [PlasmoDB: PF3D7_0930300]; Pf39 [PlasmoDB: PF3D7_1108600]; PfWLP1 [PlasmoDB: PF3D7_1443400].
The following antibodies were used in this study: mouse polyclonal antisera against PfCCp1rp1, PfCCp4rp1, and Pf39rp1 [12–14]; Pfs230 region C , Pfs48/45 (ATCC) and against the MBP-tag (Sigma-Aldrich); rabbit polyclonal antisera against PfMSP1, Pfs230 region C, PfEXP-1 ; PfGAP45 , PfGAP50 (; kindly provided by Veronique Beiss, Fraunhofer IME Aachen, Germany), PfAMA1 , alpha-tubulin (CST), and against the haemagglutinin (HA)-tag (Sigma-Aldrich). The generation of antisera against PfWLP1 is described below.
For the study, either P. falciparum wild-type (WT) strain NF54 (ATCC) or the following P. falciparum knock-out parasites were used: PfCCp1KO , PfCCp4KO , Pfs230-delta1 and Pfs230-delta2 , Pfs48/45KO . The cultures were cultivated with or without the selection drug pyrimethamine added to the culture medium (see below) in intervals of 3 weeks to remove potential revertants. The generation of the PfWLP1-HA line is described below.
Asexual blood stages and gametocytes of P. falciparum WT strain NF54 or the above listed knock-out parasite lines were cultivated in vitro in human A+ erythrocytes as described . The RPMI1640/HEPES medium (Gibco) was complemented with 10 % v/v heat-inactivated human serum, 50 µg/ml hypoxanthine (Sigma-Aldrich) and 10 µg/ml gentamicin (Gibco) and cultures were kept in an atmosphere of 5 % O2, 5 % CO2, and 90 % N2 at 37 °C. Human A+ erythrocyte sediment and serum were purchased from the Institute of Transfusion Medicine, University Hospital Aachen, Germany (PO No DKG-NT 9748). The erythrocyte and sera samples were pooled and the donors remained anonymous; the work on human blood was approved by the Ethics Commission of RWTH Aachen University. For cultivation of the knock-out parasite lines, pyrimethamine at a final concentration of 502 µM was added to the medium. To synchronize the asexual parasite blood stages, parasite cultures with 3–4 % ring stages were centrifuged, the pellet was resuspended in five times pellet’s volume of 5 % sorbitol (AppliChem)/ddH2O incubated for 10 min at room temperature (RT) . The cells were washed once with RPMI medium to remove the sorbitol, diluted to 5 % v/v hematocrit with cell culture medium and further cultivated as described above. For enrichment of gametocytes, cultures were harvested and enriched by 80/65/50/35 % v/v Percoll gradients (GE Healthcare Life Sciences) as described  and parasites were collected at the 50/35 % v/v Percoll gradient interfaces. Gametogenesis was induced by incubating mature gametocyte cultures in 100 µM xanthurenic acid dissolved in 1 % v/v 0.5 M NH4OH/ddH2O for 15–30 min at RT.
To analyze the expression of the pfwlp1 gene in asexual blood stages and gametocytes, total RNA was isolated from synchronized ring, trophozoite and schizont cultures, as well as enriched immature (stage II-IV), mature (stage V) and activated gametocytes (30 min post-activation) of WT strain NF54 using the Trizol reagent (Invitrogen) according to the manufacturer’s protocol. Following phenol/chloroform extraction and ethanol precipitation, RNA preparations were treated with RNase-free DNase I (Qiagen) to remove residual genomic DNA. RNA samples were analysed photometrically and showed A260/280 ratios higher than 2.1. The cDNA synthesis was carried out using the SuperscriptIII First-Strand Synthesis System (Invitrogen) with 2 µg of each RNA sample, following the manufacturer’s instructions. Transcript for pfwlp1 (272 bp) was amplified in 25 cycles using PfWLP1RT1 forward primer 5′-TGGGGGTTCCAAGAAGTA-3′ and PfWLP1RT1 reverse primer 5′-CGCTTATGGCTATATCTTG-3′. To confirm purity of asexual blood stage and gametocyte samples, transcripts were amplified for pfama1 (180 bp) using PfAMA1RT1 forward primer 5′-GGATTATGGGTCGATGGA-3′ and PfAMA1RT1 reverse primer 5′-GATCATACTAGCGTTCTT-3′ and for pfccp2 (187 bp) using PfCCp2RT1 forward primer 5′-TCGGATGGAGAATCCGTT-3′ and PfCCp2RT1 reverse primer 5′-GTATCCCATGTCTTGTGA-3′. Amplification of pfaldolase (378 bp) using PfAldolaseRT1 forward primer 5′-TAGATGGATTAGCAGAAAGATGC-3′ and PfAldolaseRT1 reverse primer 5′-AGAAACCAACCATCTTGAGTAGTGG-3′ was used as loading control and to test for residual genomic DNA in the negative control without reverse transcriptase. PCR products were separated by 1.2 % w/v agarose gel electrophoresis.
Recombinant protein expression
Two recombinant proteins, PfWLP1rp1 and PfWLP1rp2, spanning AA226-499 and AA294-499, respectively (regions of recombinant proteins are indicated in Fig. 1b), were expressed as fusion proteins with a N-terminal glutathione-S-transferase (GST)-tag using the pGEX-4T-1 vector (Amersham Bioscience) for PfWLP1rp1 or with a N-terminal maltose binding protein (MBP)-tag using the pIH902 vector  for PfWLPrp2. Cloning was mediated by the addition of BamHI/NotI (PfWLP1rp1) and BamHI/PstI (PfWLP1rp2) restriction sites to the ends of PCR-amplified gene fragments, using forward primer 5′-ATGGATCCATGATAGACCTAAATTATGTTAAATTG-3′ and reverse primer 5′- TAGCGGCCGCTTATCGTATTAGTGGTTTGTTTAAGCA-3′ for PfWLP1rp1 and forward primer 5′- ATGGATCCAGTTCACGTTCAAATAAATCTGAT-3′ and reverse primer 5′-TACTGCAGTTATCGTATTAGTGGTTTGTTTAAGCA-3′ for PfWLP1rp2 (restriction sites underlined). Recombinant proteins were expressed in BL21 (DE3) RIL cells according to the manufacturer’s protocol (Stratagene). Fusion proteins with respective sizes of ~58 kDa for PfWLP1rp1 and ~65 kDa for PfWLP1rp2 were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare) for PfWLP1rp1 and amylose resin (New England Biolabs) for PfWLP1rp2 according to the manufacturer’s protocols. The purity of the proteins was demonstrated by SDS-PAGE and Coomassie brilliant blue-staining (Thermo Fisher Scientific) according to the manufacturer’s protocol (Additional file 1).
Generation of mouse antisera
Recombinant fusion proteins PfWLP1rp1-GST and PfWLP1rp2-MBP were purified by affinity chromatography as stated above. PBS buffer exchange was performed prior to immunization via filter centrifugation using Amicon Ultra 15 centrifugal filter units (Sigma Aldrich). Immune sera were generated by the immunization of 6 week-old female NMRI mice (Charles River Laboratories) with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks. Mice were anesthetized 10 days after the boost by intraperitoneal injection of a mixture of ketamine and xylazine according to the manufacturer´s protocol (Sigma-Aldrich) followed by the collection of polyclonal immune sera via heart puncture. Immune sera of three mice immunized with the same antigen were pooled; sera of non-immunized mice (NMS) were collected for negative control in the experiments. The reactivity of the two antisera against the respective recombinant proteins was demonstrated via Western blotting as described below (Additional file 1). Experiments for the generation of antisera in mice were approved by the animal welfare committees of the government of Lower Franconia, Germany (ref. no. 55.2-2531.01-58/09), and of the District Council of Cologne, Germany (ref. no. 84-02.05.30.12.097 TVA).
Reverse genetics plasmid construction
A reverse genetic construct aiming for gene disruption of the pfwlp1 locus via single-crossover homologous recombination was generated using the vector pCAM-BSD [25–29]. A 404 bp fragment of the pfwlp1 locus was amplified by PCR from P. falciparum WT strain NF54 genomic DNA with gene-specific forward primer 5′-ATGGATCCCCGATATAAATAACATAAGCTAC-3′ and reverse primer 5′-TAGCGGCCGCTTACATATTATCACTCTCTGAACAGTT-3′ introducing BamHI/NotI restriction sites (underlined) in the PCR fragment, which included a stop codon. The PCR product was ligated to the BamHI/NotI-cut pCAM-BSD vector. C′-terminal double HA-tagged PfWLP1 was generated by inserting 417 bp of the homologous 3′-end of the coding gene sequence lacking a stop codon into the pCAM-BSD vector containing double HA-tag and 3′UTR from P. berghei DHFR-ts. The fragment of the 3′-end of the pfwlp1 coding sequence was amplified by PCR from P. falciparum WT strain NF54 genomic DNA with gene-specific forward primer 5′-ATCTGCAGTATGTCAAATCATACTTTAACCAT-3′ and reverse primer 5′-TAGGATCCAAAAGCCACAAACGCCCA-3′ introducing PstI/BamHI restriction sites (underlined). The PCR product was ligated to the PstI/BamHI-cut pCAM-BSD HA tagged vector.
Parasite transfection and genotype characterization
Plasmodium falciparum WT strain NF54 cultures with 4 % ring stages was electroporated with 60 µg of the respective plasmid DNA in transfection buffer as described [25–29]. Blastidicin (Invivogen) was added to a final concentration of 5.4 µM starting 4 h after transfection. Resistant parasites appeared three to 4 weeks after transfection. After 40–90 days of selection, the respective cultures were analysed for plasmid integration by diagnostic PCR. Genomic DNA of the transfected cultures was used as template in the diagnostic PCR and was isolated using the NucleoSpin Blood Kit (Macherey–Nagel) according to the manufacturer’s protocol. The following primers were used to investigate for vector integration and for the presence of episomal DNA: PfWLP1-KO-5′ integration forward primer 5′-GGGTTCCAAGAAGTACATCAA-3′ (primer 1), PfWLP1-KO-3′ integration reverse primer 5′-ACACCATCTCCTCCACCTGA-3′ (primer 2), PfWLP1-HA-5′ integration forward primer 5′-AACAAATTAGAACCCACATGGTC-3′ (primer 1), PfWLP1-HA-3′ integration reverse primer 5′-TTCCAGGAGGAACTCCAGTG-3′ (primer 2), pCAM-BSD forward primer 5′-TATTCCTAATCATGTAAATCTTAAA-3′ (primer 3) and pCAM-BSD reverse primer 5′-CAATTAACCCTCACTAAAG-3′ (primer 4). Regions of primers are indicated in Additional file 2A, C.
Pellets of non-infected human erythrocytes or synchronized schizonts and enriched non-activated gametocytes of the P. falciparum WT strain NF54 or the PfWLP1-HA line were resuspended in 0.5 % w/v saponin/0.5 % w/v NP40/PBS, homogenized and sonicated for 1 min as described . Homogenate was pelleted at 16,000 g and the supernatant was pre-purified by incubation with 5 % v/v NMS followed by 20 µl protein G-beads (Santa Cruz Biotechnology) for 30 min each at 4 °C. After centrifugation at 3400g, the supernatant was incubated for 1 h at 4 °C with 5 % v/v mouse anti-PfCCp1 antisera or rabbit antisera against the HA-tag, followed by incubation with 20 µl protein G-beads for 1 h or overnight. The beads were centrifuged, washed five times with PBS and mixed with an equal volume of SDS-loading buffer for SDS-PAGE. Precipitated proteins were analysed via Western blot as described below.
A co-immunoprecipitation assay on gametocyte lysate of the P. falciparum WT strain NF54 was conducted as described above using mouse antisera specific to PfCCp1. The charge of anti-PfCCp1 antisera used for co-immunoprecipitation was identical with the one used in a recently published co-immunoprecipitation study on the PfCCp-based protein complexes of gametocytes . Precipitated proteins were separated by SDS-PAGE followed by Silver staining using Pierce Silver Stain for Mass Spectrometry (Thermo Scientific) according to the manufacturer’s protocol. Lysate of non-infected erythrocytes was used as a negative control. Visible protein bands were cut out and an in-gel trypsin digest was performed prior to mass spectrometry analysis as described , followed by desalting and concentrating using ZipTips™ columns made from the reverse chromatography resins Poros and Oligo R3 (Applied Biosystems). Bound peptides were washed with 0.5 % w/v formic acid and eluted in 1 µl of 33 % v/v acetonitrile/0.1 % w/v trifluoroacetic acid solution saturated with α-cyano-4-hydroxycinnamic acid (Bruker Daltonics) onto a MALDI target plate and air dried before analysis in the Ultraflex-TOF TOF tandem mass spectrometer (Bruker Daltonics). Peptide mass fingerprint spectra were received in the reflectron positive mode with a pulsed extraction using ~100 laser shots. Spectra were obtained after an external calibration using reference peptides (Peptide mixture II, Bruker Daltonics). Monoisotopic masses were ascribed and processed using the software Biotools™ and FlexAnalysis™ (Bruker Daltonics) after internal calibration with trypsin autolysis peaks as internal standards (842.5100, 2211.1046 Da). Processed peptide mass fingerprints were submitted to the Mascot program  for searches against the non-redundant NCBI database with the following parameters: Taxonomy, P. falciparum and Homo sapiens; search all molecular masses and all isoelectric points; allow up to one missed proteolytic cleavage site and a peptide mass tolerance of 100 ppm. Cysteine carbamidomethylation was considered as a fixed modification and methionine oxidation as an optional modification in all the searches. Matches to human proteins were regarded as definite if the probability score was significant using the Mascot score with a p value <0.05.
Western blot analysis
Plasmodium falciparum asexual blood stage parasites of WT strain NF54 or the PfWLP1-HA line were harvested and treated with 0.15 % w/v saponin for erythrocyte lysis. Non-activated gametocytes of WT strain NF54 or of PfWLP1-HA line were enriched by Percoll gradient purification as described above. Enriched activated gametocytes were collected at 30 min post-activation. The recombinant proteins PfWLP1rp1-GST and PfWLP1rp2-MBP were affinity-purified as described above. The cell pellets or recombinant proteins were resuspended in PBS and 2× SDS-PAGE loading buffer. Proteins were separated by SDS-PAGE and transferred to Hybond ECL nitrocellulose membrane (Amersham Biosciences) according to the manufacturer’s instructions. Non-specific binding was blocked by incubation of the membranes in Tris-buffered saline containing 5 % w/v skim milk and 1 % w/v bovine serum albumin fraction V, followed by immune recognition for 2 h at RT using mouse immune sera specific for PfCCp1, Pfs230, Pf39, or rabbit antisera specific for the HA-tag or PfAMA1. Afterwards membranes were washed, incubated for 1 h at RT with a goat anti-mouse or anti-rabbit alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich). Scanned blots were processed using Adobe Photoshop CS software.
Indirect immunofluorescence assay
Parasite preparations for indirect immunofluorescence assays (IFAs) included mixed asexual blood stages and mixed gametocyte cultures as well as activated gametocyte-cultures at 15 min post-activation of WT strain NF54 or line PfWLP-1-HA. Cell monolayers were air-dried on glass slides and subsequently fixed with 4 % w/v paraformaldehyde/PBS (pH 7.4) for 10 min at RT. For membrane permeabilization and blocking of non-specific binding sites, preparations were permeabilized with 0.1 % v/v Triton X-100/125 mM glycine (Carl Roth)/PBS at RT for 30 min, followed by blocking with 3 % w/v BSA/PBS for 1 h. To liberate live gametocytes from the enveloping RBC and parasitophorous vacuole membranes, gametocytes were treated with 0.05 % w/v saponin/medium for 3 min at 37 °C prior fixation, as described in Simon et al. . In these cases, no further membrane permeabilization step was employed. Preparations were then incubated with polyclonal mouse antisera specific for Pfs230, PfCCp1, PfCCp4, and PfWLP1 or with rabbit antisera directed against the HA-tag or PfGAP50 at 37 °C for 2 h. NMS was used for negative control. Binding of primary antibody was detected by incubating the preparations with polyclonal Alexa Fluor 488-conjugated goat anti-mouse antibodies (Invitrogen Molecular Probes) at RT for 1 h. The different parasite stages or cell structures were either detected by double-labelling with stage-specific antibodies, i.e., polyclonal rabbit antisera directed against PfMSP1, PfAMA1, PfEXP1, Pfs230, PfGAP45, or alpha-tubulin, followed by incubation with polyclonal Alexa Fluor 594- or Alexa Fluor 633-conjugated goat anti-rabbit antibodies (Invitrogen Molecular Probes), or the cells were counterstained with 0.05 % w/v Evans Blue (Sigma-Aldrich)/PBS for 1 min at RT. Antisera dilutions of 1:50 to 1:1000 were used. The parasite nuclei were highlighted by incubating the specimens with Hoechst nuclear stain 33342 (Invitrogen Molecular Probes) for 1 min at RT. Labelled specimens were examined by confocal laser scanning microscopy using a Zeiss LSM 510 microscope (Additional file 3) or a Leica TCS SP5 DM6000 CFS microscope, equipped with a 20× 1.0 NA water immersion objective (Additional file 4B). Dyes were excited using the 488 nm line of an argon laser and a 633 nm HeNe laser. Otherwise the labelled specimens were investigated using an Olympus BX41 fluorescence microscope in combination with a ProgRes Speed XT5 camera (Fig. 2a, b), or using a Leica DM5500 B fluorescence microscope in combination with a Leica DFC365 FX camera (Figs. 2a–c, 3a–c, 4b, c, 5a; Additional files 5, 6, 7A). Digital images were processed using Adobe Photoshop CS software. PfWLP1 expression was quantified by determining the percentage of immune-labelled gametocytes (n = 100) in three individual experiments. Labelling of non-permeabilized and permeabilized gametocytes (n = 50) was determined in triplicate.
A WD40-repeat protein interacts with the PfCCp-based protein complex of gametocytes
Previous work demonstrated that the six secreted PfCCp proteins assemble to multimeric protein complexes that locate in the parasitophorous vacuole associated to the plasma membrane of gametocytes. The PfCCp-based protein complex is bound to the secreted large 6-cys protein Pfs230, which itself is linked to the plasma membrane via an interaction with the GPI-anchored Pfs48/45 [11–14, 32, 33]. It was the initial aim of this study to identify potential additional proteins associated with the PfCCp-based protein complex. For this purpose co-immunoprecipitation assays were performed using anti-PfCCp1 antisera as bait. Proteins precipitated from lysates of non-activated and activated gametocytes were separated by SDS-PAGE followed by silver staining; precipitates from lysates of non-infected erythrocytes were used as a negative control. By silver staining a protein running at a molecular weight of ~100 kDa was identified in the lysates of non-activated and activated gametocytes, which was absent in the erythrocyte lysate (Fig. 1a). The protein bands were excised from the gel and subjected to mass spectrometry. Peptides were assigned to a yet unknown protein of 96 kDa encoded by gene PF3D7_1443400 [34, 35]. Annotations indicated the presence of five WD40 motifs (Fig. 1b); thus the protein was termed P. falciparum WD40-repeat protein-like protein, PfWLP1.
Computational homology searches revealed that homologues of PfWLP1 are encoded in the genomes of the rodent malaria parasites Plasmodium berghei, Plasmodium chabaudi and Plasmodium yoelii, in the ape-specific species Plasmodium cynomolgi and Plasmodium reichenowi and in the human malaria parasites Plasmodium knowlesi and Plasmodium vivax (Additional file 2). Contrary to all other pwlp1 genes, pvwlp1 of P. vivax encodes for six WD40 motifs.
To confirm expression of pfwlp1 in gametocytes, diagnostic RT-PCR was conducted, using cDNA obtained from enriched immature (stage II–IV) and mature (stage V) gametocytes and from gametocytes at 30 min post-activation, further cDNA samples from purified ring stages, trophozoites and schizonts were tested for pfwlp1 expression. Diagnostic RT-PCR demonstrated transcript abundance for pfwlp1 in all of the asexual blood and gametocyte stages, which increased in schizonts (Fig. 1c). Quality of the cDNA samples was verified by monitoring transcripts of stage-specific marker proteins, i.e., pfama1 for the asexual blood stages and pfccp2 for gametocytes. Amplification of transcript encoding for the housekeeping protein pfaldolase was used as an equal-loading control (Fig. 1c). No PCR products were amplified from mock-treated RNA samples lacking reverse transcriptase, indicating that genomic DNA was absent (Additional file 3A).
PfWLP1 accumulates underneath the micronemes of merozoites and the gametocyte plasma membrane
To investigate PfWLP1 protein expression in the asexual blood and gametocyte stages, mouse antisera against two bacterially expressed peptides of PfWLP1, i.e., PfWLP1rp1 and PfWLP1rp2 (see Fig. 1b) were generated. The PfWLP1 expression pattern was determined via IFA, using anti-PfWLP1rp2 antisera. In trophozoites, highlighted by MSP1-labelling, a minor expression of PfWLP1 was observed, while the PfWLP1 signal increased during schizont maturation (Fig. 2a). In mature schizonts PfWLP1 accumulated underneath the apical pole of the forming merozoites, highlighted by labelling of the microneme transmembrane protein PfAMA1.
The localization of PfWLP1 in the maturing schizonts was investigated in more detail. Image enlargements of co-labelling experiments using anti-PfMSP1 antisera to highlight the parasite plasma membrane depict PfWLP1 in the perinuclear region (Fig. 2b); co-labelling experiments using anti-PfAMA1 antisera demonstrated PfWLP1 accumulation between apical pole and nucleus. Laser scanning confocal microscopy was used to follow the redistribution of PfWLP1 during schizont maturation and revealed that PfWLP1 is originally found underneath the PfMSP1-positive plasma membrane of the immature schizont, from where it redistributes to focal spots close to the apical pole, once the merozoites form (Additional file 3B).
PfWLP1 is further abundantly expressed in the developing gametocytes, highlighted by Pfs230-labelling; here PfWLP1 labelling was found to be both intracellular and plasma membrane-associated (Fig. 3a). A total of 94 ± 3 % of Pfs230-labelled gametocytes were also positive for PfWLP1 (n = 100, three individual experiments), indicating that PfWLP1 is expressed by gametocytes of both genders. In mature gametocytes, the peripheral labelling often disappeared and the presence of PfWLP1 was then restricted to the cytosol. PfWLP1 expression was further detected in macro- and microgametes (Fig. 3a). When NMS was used in the IFAs, no labelling was detected in the asexual blood stages or gametocytes (Additional file 4A). When anti-PfWLP1rp1 antisera were used in the IFAs, a similar labelling pattern was observed in schizonts and gametocytes (Additional file 4B).
Because in developing gametocytes the majority of PfWLP1 is located close to the plasma membrane, co-localization experiments were performed, using antisera against proteins located in close proximity to the plasma membrane, i.e., the peripheral protein Pfs230, the inner membrane complex protein PfGAP45 and the cytoskeletal protein alpha-tubulin. The IFAs demonstrated that PfWLP1 is localized in close proximity to the inner membrane complex and image enlargement revealed that PfWLP1 is located underneath the Pfs230-positive plasma membrane (Fig. 3b, c). Further, PfWLP1 co-localized with alpha-tubulin in the maturing schizonts and gametocytes (Additional file 5). Noteworthy, in the mature stage V gametocytes, when the tubulin network underneath the plasma membrane disassembles (, reviewed in ), the rim-associated PfWLP1 labelling also disappeared. Because PfWLP1 is also expressed in lines PfCCp1KO, PfCCp4KO, Pfs230-delta1, Pfs230-delta2, and Pfs48/45KO (Additional file 6), the expression of PfWLP1 is not dependent on the presence of any of the components of the PfCCp-based multimeric protein complex.
PfWLP1 is crucial for erythrocytic schizogony
For functional studies the pfwlp1 gene locus was targeted for disruption via single cross-over homologous recombination, using the pCAM-BSD vector [25–29]. The pfwlp1-KO vector contained an insert encoding the N-terminal portion of the protein, as well as a cassette conferring resistance to blasticidin. Integration into the respective pfwlp1 gene would result in a disrupted (pseudo-diploid) locus (Additional file 7A).
The pCAM-BSD-based pfwlp1-KO vector was electroporated into ring-stage parasites and populations of blasticidin-resistant parasites were obtained. However, these parasites contained only non-integrated episomes and no integration of the respective pfwlp1-KO vector was detected by diagnostic PCR (Additional file 7B) even after prolonged culturing (24 weeks).
To verify that the pfwlp1 locus was accessible for recombination, a pCAM-BSD-based vector that contained an insert encoding the 3′-end of PfWLP1 fused to the sequence encoding a double HA-tag, followed by the 3′-untranslated region from the P. berghei dhfr-ts gene, was generated (Additional file 7C). Integration of the vector would result in a complete pfwlp1 gene followed by a 3′-located HA-sequence. This recombination is predicted not to cause loss of function of the gene product, but to generate a functional protein.
The pfwlp1-HA vector was electroporated into ring-stage cultures and treated with blasticidin as described above. DNA was isolated from blasticidin-resistant HA-tag vector populations ~6–8 weeks following electroporation and PCR revealed that integration of the vector had occurred. Following clonal dilution, the pfwlp1-HA clone 1A7 was isolated (Additional file 7D).
Expression of HA-tagged PfWLP1 was confirmed via Western blotting, using anti-HA antibodies. In the PfWLP1-HA line, HA-tagged PfWLP1 was observed in lysates of enriched ring stages, trophozoites and schizonts, as well as in cultures of immature and mature gametocytes and in gametocytes at 30 min post-activation. In all lysates, PfWLP1-HA was running at a predicted molecular weight of ~108 kDa (PfWLP1 ~96 kDa; HA-tag ~12 kDa) (Fig. 4a). No protein bands were detected when lysates of non-infected erythrocytes or lysates of WT asexual blood stages and gametocytes were immunoblotted with the anti-HA antibody. Immunolabelling with anti-Pf39 was used as a loading control (Fig. 4a).
IFAs confirmed the expression of PfWLP1-HA in schizonts and merozoites of the PfWLP1-HA line, and the protein initially localized underneath the plasma membrane and then accumulated underneath the apical poles of the forming merozoites (Fig. 4b). Further, PfWLP1-HA was found in the developing gametocytes of the PfWLP1-HA line (Fig. 4c). Consistent with the PfWLP1 location in WT parasites, the HA-tagged protein was present both in the cytoplasm and associated to the gametocyte plasma membrane. In mature gametocytes, the peripheral labelling disappeared. PfWLP1-HA was further observed associated to the surface of the emerging gametes (Fig. 4c). IFAs further confirmed a co-labelling, when antibodies directed against PfWLP1 (PfWLP1rp2) and the HA-tag were used (Additional file 8A). Also, PfWLP1-HA was detectable, when the protein was immunoprecipitated from a lysate of the PfWLP1-HA line, using anti-PfWLP1rp2 antisera, and when the precipitate was subsequently immunoblotted with anti-HA antibody (Additional file 8B).
To confirm that PfWLP1 is an intracellular protein that localizes underneath the gametocyte plasma membrane, live gametocytes were collected and liberated from the enveloping RBC and parasitophorous vacuole membranes via mild saponin-treatment as previously described . The gametocytes were subsequently fixed with paraformaldehyde, one part of the fixed cells was then membrane-permeabilized, while the other portion was not membrane-permeabilized. Subsequent IFAs showed a labelling for the peripheral plasma membrane-bound protein Pfs230 in non-permeabilized gametocytes, but neither a labelling for the inner membrane complex protein PfGAP50 or for PfWLP1 was observed these cells, indicating that PfWLP1, like PfGAP50, is located intracellularly and thus not accessible for the antibodies in the IFAs. Also no labelling was detected for the HA-tag, when saponin-treated non-membrane permeabilized gametocytes of the PfWLP1-HA line were used (Fig. 5a). Furthermore, quantification of non-permeabilized gametocytes showed that only minor proportions are labelled for the intracellular protein PfGAP50 (5.3 ± 2.4 %) or for PfWLP1 (9.3 ± 2.5 %) and PfWLP1-HA (6 ± 3.2 %). In contrast 99.3 ± 0.94 % of non-permeabilized gametocytes showed labelling for the membrane-bound protein Pfs230 (n = 50; in triplicate) (Fig. 5b). When the membranes were permeabilized and thus were accessible for the antibodies, all of the gametocytes labelled for the respective proteins (Fig. 5a, b).
PfWLP1 associates with components of adhesion protein complexes
In a final set of experiments the PfWLP1-HA-expressing line was used to investigate, if PfWLP1 is part of larger adhesion protein complexes of merozoites and gametocytes. Firstly, PfWLP1-HA was immunoprecipitated from schizont-enriched lysate, using anti-HA antibody. The precipitate was subjected to SDS-PAGE followed by Western blotting. Immunoblotting detected PfAMA1 (~72 kDa) in the precipitate, when the respective antibody was used (Fig. 6a). Subsequently, lysates of enriched gametocytes and of gametocytes at 30 min post-activation were used in the assays. By means of immunoblotting, Pfs230 and PfCCp1 (~363 and 185 kDa, respectively) were detected in the precipitates (Fig. 6b). Immunoblotting with anti-HA antibody verified that the HA-tagged PfWLP1 was present in the precipitates, which was running with a molecular weight of ~108 kDa. Immunoblotting with anti-Pf39 antibody, which was used as a negative control in both experiments, did not detect any protein bands (Fig. 6a, b). Neither were protein bands specific for PfAMA1, Pfs230, PfCCp1 or PfWLP1-HA detected in lysates of non-infected erythrocytes (Fig. 6a, b). The co-immunoprecipitation data indicate that PfWLP1 associates with components of adhesion protein complexes of merozoites and gametocytes.
WD40-repeat proteins belong to one of the most abundant protein classes in eukaryotic organisms, involved in a variety of cellular processes, such as signal transduction, cytoskeleton assembly, or cell cycle control. They all share a common function by acting as a scaffold for protein–protein interactions, thereby mediating the formation of protein complexes and in consequence coordinating downstream events ([38, 39], reviewed in [40, 41]).
The WD40 repeats are short repeating ~40–66 amino acid motifs, terminated by an eponymous tryptophan-aspartate (WD) dipeptide [38, 39]. Usually seven or multiple of seven WD40-repeats compose a WD40 domain, which is folded into a seven-bladed ß-propeller with a funnel-like shape, serving with the circumference as three main interaction sites. WD40 domains can act as a large interaction platform in cellular interaction networks; WD40-repeat proteins can thus be involved in various multi-protein complexes with different functions. Noteworthy, each WD40-repeat protein can possess multiple functions depending on its direct and indirect interactions partners ([42, 43]; reviewed in ).
A recent in silico analysis of the P. falciparum genome revealed 80 putative WD40-repeat proteins; these included 15 proteins specific to the genus Plasmodium . In the same study, protein–protein interaction network analyses indicated more than 1900 potential interactions, and only nine of the plasmodial WD40-repeat proteins appear to be stage-specific.
The majority of plasmodial WD40-repeat proteins are, to date, of unknown functions and only two of them, PfSec13 and PfRACK, have hitherto been studied in detail. In humans, Sec13 is known to be involved in the biogenesis of COPII-coated vesicles at the endoplasmic reticulum, whereas it also is an essential component in the nuclear pore complex . PfSec13 has been described as an unusual component of the parasite nuclear pore complex involved in several putative cellular processes, such as chromatin regulation or vesicle biogenesis . Another WD40-repeat protein characterized in Plasmodium is the receptor for activated C kinases (RACK) . In humans, RACK proteins act as a scaffold for activated protein kinase C and other proteins, thereby stabilizing the active enzymes and increasing the substrate phosphorylation, and are further involved in integrin-dependent cell migration [48, 49]. In P. falciparum, PfRACK is expressed during the erythrocytic replication cycle, where it is exported into the erythrocyte cytosol and able to inhibit mammalian Ca2+ signals thereby utilizing the host cell signalling machinery [47, 50].
The here-described PfWLP1 is expressed in the maturing schizonts, where it is initially found underneath the plasma membrane and then relocalizes underneath the micronemes, once the merozoites have formed. PfWLP1 is further expressed in the developing gametocytes, where it accumulates at the sub-pellicular region. Some of the complex partners of PfWLP1 were identified, which in merozoites includes the micronemal transmembrane protein PfAMA1, while in gametocytes PfWLP1 associates with Pfs230 and PfCCp1, two components of the PfCCp-based protein complex. Further, PfWLP1, which lacks any signal peptide, is an intracellular protein. For PfAMA1, a direct interaction between PfWLP1 and the C-terminal intracellular part of the transmembrane protein might be possible. The interaction between PfWLP1 and peripheral proteins Pfs230 and PfCCp1, though, has to be indirect and would have to be mediated by yet unknown membrane-spanning proteins. In view of the data that PfWLP1 is found in close proximity with sub-pellicular proteins like PfGAP45, PfGAP50 or tubulin, larger protein complexes containing PfWLP1 might connect adhesion proteins like PfAMA1 or the PfCCp proteins with structural elements of the parasite, e.g. the inner membrane complex or the cytoskeleton. Molecular connections between plasma membrane-associated adhesion proteins and structural elements are already known for the actin-myosin motor complex of the parasite invasive stages, where aldolase connects distinct transmembrane proteins of the TRAP family with the actin-myosin motor, while PfGAP45/PfGAP50 connect the actin-myosin motor with the outer membrane of the inner membrane complex (reviewed in ).
This is the first report on a plasmodial WD40-repeat protein associating with cell adhesion protein complexes of the P. falciparum blood stages. The here presented data lead to the hypothesis that PfWLP1 is involved in the stability or anchoring of membrane-linked adhesion protein complexes in schizonts and gametocytes, which, via larger protein complexes, might be connected to sub-pellicular or cytoskeletal elements of the parasite. Further analysis of the function of PfWLP1 might reveal the detailed mode of action and deepen the knowledge on the role of WD40-repeat proteins in malaria parasites.
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AvB carried out the diagnostic RT-PCR, immunoassays, co-immunoprecipitation, in silico analysis, participated in reverse genetics studies and helped to draft the manuscript. AK carried out the recombinant protein expression, mouse antisera generation, reverse genetics studies and participated in the immunoassays. NS participated in co-immunoprecipitation assays and mouse antisera generation. VNN participated in recombinant protein expression, mouse antisera generation and the reverse genetics studies. SM conducted selected confocal microscopy analyses. SB and JMP carried out the mass spectrometric analysis and gene annotation. RF participated in the study coordination. GP designed and coordinated the study and drafted the manuscript. All authors read and approved the final manuscript.
We thank Ludmilla Sologub (University of Würzburg) for technical assistance and Veronique Beiss (Fraunhofer IME Aachen) for providing rabbit anti-PfGAP50 antibody. This research was funded by grants PR905/1-3, PR905/4-1 and SFB479/B8 from the Deutsche Forschungsgemeinschaft (to GP). The work was additionally supported by MALSIG of the EU 7th framework program fund (to GP). NS received a fellowship from the program for female scientists of the University of Würzburg. AK was an associated member of the BioMedTec International Graduate School of Science “Lead structures of cell function” of the Elite Network Bavaria.
The authors declare that they have no competing interests.
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Bohl, A., Kuehn, A., Simon, N. et al. A WD40-repeat protein unique to malaria parasites associates with adhesion protein complexes and is crucial for blood stage progeny. Malar J 14, 435 (2015) doi:10.1186/s12936-015-0967-x
- Plasmodium falciparum
- PfCCp protein