Fig. 6

PfWLP1 associates with cell adhesion proteins of merozoites and gametocytes. a Co-immunoprecipitation of PfWLP1 with PfAMA1. Lysates of enriched schizonts of line PfWLP1-HA were immunoprecipitated with anti-HA antisera, followed by Western blot analysis using antisera directed against PfAMA1 (~72 kDa). b Co-immunoprecipitation of PfWLP1 with Pfs230 and PfCCP1. Lysates of non-activated and activated gametocytes of line PfWLP1-HA were immunoprecipitated with anti-HA antisera, followed by Western blot analysis using antisera directed against Pfs230 or PfCCp1 (360 and 185 kDa, respectively). Immunoblotting with anti-HA antisera confirmed the presence of PfWLP1-HA (~108 kDa) in all precipitates. A smeared protein band migrating at ~55 kDa resembled the heavy chain of the rabbit antibody. Erythrocyte lysate (Ec) and immunoblotting with mouse antisera directed against Pf39 (39 kDa) were used as a negative controls. Asterisks indicate the precipitated proteins. Data are representative of two to three independent experiments