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Fig. 4 | Malaria Journal

Fig. 4

From: Beninese children with cerebral malaria do not develop humoral immunity against the IT4-VAR19-DC8 PfEMP1 variant linked to EPCR and brain endothelial binding

Fig. 4

a Adhesion profile of IEs selected with anti-VAR19 antibodies (FCR3-VAR19), EPCR (FCR3-EPCR) and human brain endothelial cells (FCR3-HBEC5i). Data shown is the mean number of IEs per mm2 adhering to receptors coated on plastic Petri dishes, as determined in three independent assays in duplicated spots (error bars represents the standard deviation). b Transcription analysis of var genes expressed at the same parasite cycle as the adhesion assays were done. c Flow cytometry analysis of IEs obtained after panning labelled with rabbit anti-VAR19, anti-VAR2CSA and mouse anti-IgM antibodies (blue histograms) compared to negative control (without primary antibody, red histograms). d Immunofluorescence assay of FCR3-VAR19 IEs stained with rabbit anti-VAR19 IgG (red). The parasite nucleus is stained with Hoechst 3342 (blue)

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