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Table 1 Limits of detection of Plasmodium falciparum under varied storage conditions of Plasmodium falciparum and Plasmodium vivax admixtures

From: An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples

 

Sample collection storage conditions

Admixture of P. falciparum and P. vivax

 

3 days at 22 °C

Frozen (−80 °C)

3 days at 28 °C 80 % RH

7 or 14 days at 28 °C 80 % RH

Pf only

Pv only

Pf w/Pv plasmida

Pv plasmid w/Pfb

Pf LoD (p/mL)

20.5

23.3

20.5

<16

21.3

–

25.1

–

Pv LoD (copies/μL)

–

–

–

–

–

19.7

–

49.5

  1. LoD Limits of detection; Pf P. falciparum; Pv P. vivax; RH relative humidity
  2. a3.8 × 104 plasmid copies/μL
  3. b1 × 104 p/mL P. falciparum
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Malaria Journal

ISSN: 1475-2875

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