Table 1 Primers and sequences used for nested PCR

Primary

rPLU6

5′TTAAAATTGTTGCAGTTAAAACG3′

rPLU5new

5′CYTGTTGTTGCCTTAAACTTC3′

P. falciparum

rFAL1

5′TTAAACTGGTTTGGGAAAACCAAATATATT3′

rFAL2

5′ACACAATAGACTCAATCATGACTACCCGTC3′

P. malariae

rMAL1

5′ATAACATAGTTGTACGTTAAGAATAACCGC3′

rMAL2

5′AAAATTCCCATGCATAAAAAATTATACAAA3′

P. vivax

rVIV1

5′CGCTTCTAGCTTAATCCACATAA3′

rVIV2

5′ACTTCCAAGCCGAAGCAAAGAAA 3′

MSP2 PCR

 Primary

S2-Fw

5′GAA GGT AAT TAA AAC ATT GTC3′

S3-REv

5′GAG GGA TGT TGC TGC TCC ACA G3′

 Nested

S1_Tail

5′GCT TAT AAT ATG AGT ATA AGG AGA A3′

M5

5′-6FAM-GCA TTG CCA GAA CTT GAA3′

N5

5′-VIC-CTG AAG AGG TAC TGG TAG A3′