Fig. 6
(His)6-Pf14-3-3I interaction with GST-PfAKAL and GST-PfPKA-R. a Anti-polyhistidine western blot analysis after incubation of GST-PfAKAL beads in (His)6-Pf14-3-3I solution. GST beads (1), molecular marker (2), flowthrough after incubation of GST-PfAKAL beads in the (His)6-Pf14-3-3I solution (3), glutathione agarose bead (4), and GST-PfAKAL beads (5), incubated in (His)6-Pf14-3-3I solution, (His)6-Pf14-3-3I (6). b PfAKAL and Pf14-3-3I localizations by immuno-staining of 3D7 parasites infected erythrocytes smears with mouse anti-PfAKAL (red) and rat anti-Pf14-3-3I (green) antibodies. The merge shows colocalization of the two proteins (yellow). c Deconvolution microscopy analysis (Imaris software) on a trophozoite parasite showing PfAKAL (red) and Pf14-3-3I (green) localizations in the infected red blood cell, nucleus staining (blue). The pink and yellow colors represent the localizations where blue and red signals or green and red signals are merged respectively. The bars represent 2 μm, d Anti-polyhistidine western blot analysis after incubation of GST-PfPKA-R beads in (His)6-Pf14-3-3I solution. (His)6-Pf14-3-3I (1); GST beads in (His)6-Pf14-3-3I solution (2); molecular marker (3); GST-PfAKAL beads in (His)6-Pf14-3-3I solution (4); GST-PfPKA-R beads in (His)6-Pf14-3-3I solution (5). e Gel filtration analysis of (His)6-Pf14-3-3I showing two pics: pic 1 corresponds to a dimer of (His)6-Pf14-3-3I and pic2 corresponding to a monomer of (His)6-Pf14-3-3I