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Fig. 7 | Malaria Journal

Fig. 7

From: Characterization of an A-kinase anchoring protein-like suggests an alternative way of PKA anchoring in Plasmodium falciparum

Fig. 7

Phosphorylation of (His)6-PfPKA-R, (His)6-Pf14-3-3I or (His)6-PfAKAL by bovine PKA catalytic subunit (bPKA). Radiolabel kinase activity assays were deployed to detect phosphorylation of the proteins. Assays without PKA or with H89, a potent PKA inhibitor were used as negative controls. Coomassie blue staining (b) of radiolabelled SDS-gels (a) was used as a loading control

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