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Table 1 Culture parameters of the experiments

From: Benchmarking vector arthropod culture: an example using the African malaria mosquito, Anopheles gambiae (Diptera: Culicidae)

Treatment

Starting number (L1s)

Liquid Volume

(ml)

Density

(larvae /ml)

Feeding schedule

Diet volume

(ml)

Diet concentration % w/v

Low diet

128

30

4.3

Alternate days

0.64

0.5

Medium diet

128

30

4.3

Alternate days

0.64

1.0

High diet

128

30

4.3

Alternate days

0.64

2.0

SOP

750

175

4.3

According to the SOP

According to the SOP

2.0

  1. * Feeding regime according to the SOP (Additional file 1) as follows - Days 1 and 3: 5ml, Day 4: 7ml, Day 5: 10ml, Days 6 and 7: 10-12ml, thereafter: by judgement