Defining rules of CD8+ T cell expansion against pre-erythrocytic Plasmodium antigens in sporozoite-immunized mice

Table 2 Comparison of immunogenicity data to available mass spectrometry-based expression data

Antigen	Epitope	MHC	Protective?	Conserved in Pb/Py?	Recall?		No. spectra in Py/Pf spz^b (Py rank/Pf rank)	No. spectra from Py/Pf spz surface?^b (Py rank/Pf rank)	Δ Transcription (24/40 h LS vs sgSPZ)^c
Antigen	Epitope	MHC	Protective?	Conserved in Pb/Py?	Hom	Het	No. spectra in Py/Pf spz^b (Py rank/Pf rank)	No. spectra from Py/Pf spz surface?^b (Py rank/Pf rank)	Δ Transcription (24/40 h LS vs sgSPZ)^c
PyCSP	SYVPSAEQI	H2K^d	Yes	No^a	Yes	NA	549/1460 (22/5)	166/140 (1/1)	ND/ND
PbTRAP	SALLNVDNL	H2D^b	Yes	No^a	Yes	NA	1437/2164 (13/4)	16/1 (10/22)	−1.91/−3.01
PbS20	VNYSFLYLF	H2 K^b	No	Yes	No	Yes	536/652 (61/44)	ND/5 (NA/11)	ND/ND
PyL3	GYKSGMSHI	H2K^d	No	Yes	No	Yes	291/82 (89/402)	ND/ND (NA/NA)	3.00/2.88
PbGAP50	SQLLNAKYL	H2D^b	No	Yes	No	No	390/584 (51/24)	ND/ND (NA/NA)	1.31/–1.08
PbF4	EIYIFTNI	H2^b	No	Yes	No	No	46/84 (630/481)	ND/ND (NA/NA)	ND/ND
PbNCY	NCYDFNNI	H2^b	No	No^d	No	No	ND/ND (NA/NA)	ND/ND (NA/NA)	ND/ND

Hom homologous; Het heterologous; NA not applicable; ND not detected
^aPyCSP SYVPSAEQI vs. PbCSP SYIPSAEKI; PyTRAP SALLVVDTL vs. PbTRAP SALLNVDNL, amino acid differences underlined
^bNumber of spectra detected for Py (P. yoelii) and Pf (P. falciparum) orthologues of the tested antigen based on mass spectrometry of sporozoites as described in [45]
^cChange in mRNA transcription for 24 h LS:sgSPZ or 40 h LS:sgSPZ based on [47] and PlasmoDB [42]
^dPy protein truncated compared to Pb protein

ISSN: 1475-2875