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Table 1 List of primers used in this study

From: Development of a Plasmodium berghei transgenic parasite expressing the full-length Plasmodium vivax circumsporozoite VK247 protein for testing vaccine efficacy in a murine model

Locus

Primer name

Sequence (5′–3′)

Purpose

PvCSP(VK247)

pPvCSP-VK247-F4

CACCCGGGCACAATGTAGATCTGTCCAAGGCCATA

Generation of PvCSP(VK247)/Pb

pPvCSP-VK247-R1

CAATTGAACTATTTACGACATTAAACACACTGGAACACTTATCCATTGTACAAACATCAGTCTCAAGGTCATTCAA

pPvCSP-VK247-F1

CACCCGGGAATGTCGTAAATAGTTCAATTGGATTAATAATGGTA

pPfCSP-R7

GTCGACTGTTAAATGAACTTCGAAGAATTCATTTTTTGTG

PbCSP

pPbCSP-F3

ATGAAGAAGTGTACCATTTTAGTTGTAGCG

Confirmation of integration by recombination for PvCSP genes

pPbCSP-R3

TACAAATCCTAATGAATTGCTTACAATATT

PvCSP(VK210)

pPvCSP-VK210-F4

CACCCGGGCACAATGTAGATCTGTCCAAGGCCATA

pPvCSP-VK210-R1

TCCATCTGCTCTGTCTCCTGGTTG

PvCSP(VK247)

pPvCSP-VK247-F4

CACCCGGGCACAATGTAGATCTGTCCAAGGCCATA

pPvCSP-VK247-R3

ATTGCCAGCCCCATTTGCTCCTGGTTG

Pb 18 s rRNA

p18 s rRNA-F1

AAGCATTAAATAAAGCGAATACATCCTTAC

Confirmation of integration by recombination for PvCSP genes and invasion assay

p18 s rRNA-R1

GGAGATTGGTTTTGACGTTTATGTG

Human action

pHA067803-F

TGGCACCCAGCACAATGAA

Invasion assay

pHA067803-R

CTAAGTCATAGTCCGCCTAGAAGCA